远程缺血预处理对自发性高血压大鼠的降压作用。

The antihypertensive effect of remote ischemic conditioning in spontaneously hypertensive rats.

机构信息

Department of Neurology, Aerospace center Hospital, Beijing, China.

Beijing Institute of Brain Disorder, Capital Medical University, Beijing, China.

出版信息

Front Immunol. 2023 Jan 12;13:1093262. doi: 10.3389/fimmu.2022.1093262. eCollection 2022.

DOI:10.3389/fimmu.2022.1093262

PMID:36713422

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9878686/

Abstract

PURPOSE

Limb remote ischemic conditioning (LRIC) may be an effective method to control hypertension. This study investigated whether LRIC decreases blood pressure by regulating the hypertensive inflammatory response in spontaneously hypertensive rats (SHR).

METHOD

The SHR and aged-matched Wistar rats with different ages were randomly assigned to the SHR group, SHR+LRIC group, Wistar group, and Wistar + LRIC group. LRIC was conducted by tightening a tourniquet around the upper thigh and releasing it for three cycles daily (10 mins x3 cycles). Blood pressure, the percentage of monocytes and T lymphocytes, and the concentration of pro-inflammatory cytokines in the blood were analyzed.

RESULTS

The blood pressure of SHR was significantly higher than that of age-matched Wistar rats. LRIC decreased blood pressure in SHR at different ages (4, 8, and 16 weeks old), but had no effect on the blood pressure in Wistar rats. Flow cytometry analysis showed that blood monocytes and CD8 T cells of SHR were higher than those of Wistar rats. LRIC significantly decreased the percentage of monocytes and CD8 T cells in SHR. Consistent with the changes of immune cells, the levels of plasma IL-6 and TNF-α in SHR were also higher. And LRIC attenuated the plasma IL-6 and TNF-α levels in SHR.

CONCLUSION

LRIC may decreased the blood pressure via modulation of the inflammatory response in SHR.

摘要

目的

肢体远端缺血预处理（LRIC）可能是控制高血压的有效方法。本研究探讨了 LRIC 是否通过调节自发性高血压大鼠（SHR）的高血压炎症反应来降低血压。

方法

将不同年龄的 SHR 和年龄匹配的 Wistar 大鼠随机分为 SHR 组、SHR+LRIC 组、Wistar 组和 Wistar+LRIC 组。LRIC 通过每天收紧大腿上的止血带并释放三次（每次 10 分钟 x3 次）来进行。分析血压、单核细胞和 T 淋巴细胞的百分比以及血液中促炎细胞因子的浓度。

结果

SHR 的血压明显高于年龄匹配的 Wistar 大鼠。LRIC 降低了不同年龄（4、8 和 16 周龄）的 SHR 血压，但对 Wistar 大鼠的血压没有影响。流式细胞术分析显示，SHR 的血液单核细胞和 CD8 T 细胞高于 Wistar 大鼠。LRIC 显著降低了 SHR 中的单核细胞和 CD8 T 细胞的百分比。与免疫细胞的变化一致，SHR 血浆中 IL-6 和 TNF-α的水平也较高。LRIC 减轻了 SHR 血浆中 IL-6 和 TNF-α的水平。