大鼠脂肪组织中的5α-还原酶活性。

5 alpha-reductase activity in rat adipose tissue.

作者信息

Zyirek M, Flood C, Longcope C

机构信息

Department of Obstetrics/Gynecology, University of Massachusetts Medical School, Worcester 01605.

出版信息

Proc Soc Exp Biol Med. 1987 Nov;186(2):134-8. doi: 10.3181/00379727-186-42592.

DOI:10.3181/00379727-186-42592

PMID:3671352

Abstract

We measured the 5 alpha-reductase activity in isolated cell preparations of rat adipose tissue using the formation of [3H]dihydrotestosterone from [3H]testosterone as an endpoint. Stromal cells were prepared from the epididymal fat pad, perinephric fat, and subcutaneous fat of male rats and from perinephric fat of female rats. Adipocytes were prepared from the epididymal fat pad and perinephric fat of male rats. Stromal cells from the epididymal fat pad and perinephric fat contained greater 5 alpha-reductase activity than did the adipocytes from these depots. Stromal cells from the epididymal fat pad contained greater activity than those from perinephric and subcutaneous depots. Perinephric stromal cells from female rats were slightly more active than those from male rats. Estradiol (10(-8) M), when added to the medium, caused a 90% decrease in 5 alpha-reductase activity. Aromatase activity was minimal, several orders of magnitude less than 5 alpha-reductase activity in each tissue studied.

摘要

我们以[3H]睾酮生成[3H]二氢睾酮作为终点，测定了大鼠脂肪组织分离细胞制剂中的5α-还原酶活性。基质细胞取自雄性大鼠的附睾脂肪垫、肾周脂肪和皮下脂肪以及雌性大鼠的肾周脂肪。脂肪细胞取自雄性大鼠的附睾脂肪垫和肾周脂肪。附睾脂肪垫和肾周脂肪的基质细胞所含5α-还原酶活性高于这些部位的脂肪细胞。附睾脂肪垫的基质细胞比肾周和皮下部位的基质细胞活性更高。雌性大鼠肾周基质细胞的活性略高于雄性大鼠。当向培养基中添加雌二醇（10(-8) M）时，5α-还原酶活性降低90%。在所研究的每个组织中，芳香化酶活性极低，比5α-还原酶活性低几个数量级。