转录组分析涉及蓝色和白色花朵颜色变化的关键基因。

Transcriptome Analysis of Key Genes Involved in Color Variation between Blue and White Flowers of .

机构信息

Flower Research Institute of Yunnan Academy of Agricultural Sciences, National Engineering Research Center For Ornamental Horticulture, Key Lab of Yunnan Flower Breeding, Kunming 650205, China.

College Horticulture of Nanjing Agricultural University, Nanjing 210095, China.

出版信息

Biomed Res Int. 2023 Jan 18;2023:7407772. doi: 10.1155/2023/7407772. eCollection 2023.

DOI:10.1155/2023/7407772

PMID:36714023

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9876678/

Abstract

Dykes (Southwest iris) is an extensively distributed Iridaceae species with blue or white flowers. Hereby, we performed a systematic study, employing metabolomics and transcriptomics to uncover the subtle color differentiation from blue to white in Southwest iris. Fresh flower buds from both cultivars were subjected to flavonoid/anthocyanin and carotenoid-targeted metabolomics along with transcriptomic sequencing. Among 297 flavonoids, 24 anthocyanins were identified, and 13 showed a strong down-accumulation pattern in the white flowers compared to the blue flowers. Significant downregulation of and genes involved in the glycosylation of anthocyanins was predicted to hinder the accumulation of anthocyanins, resulting in white coloration. Besides, no significant altered accumulation of carotenoids and expression of their biosynthetic genes was observed between the two cultivars. Our study systematically addressed the color differentiation in flowers, which can aid future breeding programs.

摘要

蝴蝶花（西南鸢尾）是一种广泛分布的鸢尾科植物，花朵呈蓝色或白色。在此，我们采用代谢组学和转录组学方法进行了系统研究，以揭示西南鸢尾从蓝色到白色的微妙颜色分化。我们对两个品种的新鲜花蕾进行了类黄酮/花青素和类胡萝卜素靶向代谢组学以及转录组测序。在 297 种类黄酮中，鉴定出 24 种花青素，其中 13 种在白色花朵中的积累量明显低于蓝色花朵。预测和基因的下调表达，这些基因参与花青素的糖基化，可能阻碍了花青素的积累，导致白色着色。此外，在两个品种之间没有观察到类胡萝卜素的显著积累和生物合成基因的表达变化。我们的研究系统地解决了花的颜色分化问题，这有助于未来的育种计划。