Faculty of Health and Medical Sciences, Section for Oral, Biology and Immunopathology, Department of Odontology, University of Copenhagen, Copenhagen, Denmark.
Institute for Inflammation Research, Center for Rheumatology and Spine Diseases, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark.
J Periodontol. 2023 Aug;94(8):997-1007. doi: 10.1002/JPER.22-0438. Epub 2023 Mar 5.
Cytokine-producing B cells play a well-established role in modifying immune responses in chronic inflammatory diseases. We characterized B-cell cytokine responses against periodontitis-associated bacteria in patients with periodontitis.
Blood and saliva samples were collected from patients with periodontitis grade B (N = 31) or grade C (N = 25), and 25 healthy controls (HCs). Mononuclear cells were stimulated with Porphyromonas gingivalis, Fusobacterium nucleatum, Staphylococcus epidermidis, or Cutibacterium acnes, and B-cell production of tumor necrosis factor (TNF)-α, interleukin (IL)-6, interferon (IFN)-γ, IL-10 and transforming growth factor (TGF)-β by B cells was assessed by flow cytometry.
HCs had higher baseline frequencies of B cells producing IFN-γ or TNF-α than grade B patients, but only B cells from grade B patients showed significant differentiation into IFN-γ-, TNF-α-, TGF-β-, or IL-10-producing cells after challenge with P. gingivalis and into IFN-γ-, TGF-β-, or IL-10-producing cells after challenge F. nucleatum. Notably, the baseline frequency of IL-10-producing B cells from grade C patients correlated inversely with clinical attachment loss (AL). The major proportion of the IFN-γ- and TGF-β-producing B cells were CD27 memory cells, while the IL-10-producing B cells were mainly CD27 CD5 .
B cells from grade B patients, particularly those harboring P. gingivalis, showed proinflammatory B-cell responses to P. gingivalis. Moreover, the baseline frequency of IL-10-producing B cells in the grade C group correlated inversely with AL, suggesting a diminished immunoregulatory capacity of IL-10-producing B cells in these patients.
产生细胞因子的 B 细胞在慢性炎症性疾病中调节免疫反应方面发挥着重要作用。我们对牙周炎患者针对牙周炎相关细菌的 B 细胞细胞因子反应进行了特征描述。
从牙周炎 B 级（N=31）或 C 级（N=25）患者以及 25 名健康对照者（HCs）中采集血液和唾液样本。单核细胞用牙龈卟啉单胞菌、核梭杆菌、表皮葡萄球菌或痤疮丙酸杆菌刺激，通过流式细胞术评估 B 细胞产生肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6、干扰素（IFN）-γ、IL-10 和转化生长因子（TGF）-β的情况。
HCs 产生 IFN-γ或 TNF-α的 B 细胞基线频率高于 B 级患者，但只有 B 级患者的 B 细胞在受到牙龈卟啉单胞菌和核梭杆菌的刺激后才会显著分化为 IFN-γ、TNF-α、TGF-β或 IL-10 产生细胞。值得注意的是，C 级患者产生 IL-10 的 B 细胞的基线频率与临床附着丧失（AL）呈负相关。IFN-γ和 TGF-β产生 B 细胞的主要比例是 CD27 记忆细胞，而 IL-10 产生 B 细胞主要是 CD27 CD5 。
B 级患者的 B 细胞，尤其是携带牙龈卟啉单胞菌的 B 细胞，对牙龈卟啉单胞菌表现出促炎 B 细胞反应。此外，C 级组中产生 IL-10 的 B 细胞的基线频率与 AL 呈负相关，提示这些患者的 IL-10 产生 B 细胞的免疫调节能力下降。
