Beard Andrew, Oramas Rebecca, Sun Jianjun
Department of Physiology & Neurobiology, University of Connecticut, Storrs, CT, USA.
Institute for Systems Genomics, University of Connecticut, Storrs, CT, USA.
Methods Mol Biol. 2023;2626:253-276. doi: 10.1007/978-1-0716-2970-3_13.
Ovulation is a critical reproductive process by which a mature oocyte is released from the ovary for fertilization. This process requires the coordination of multiple cellular and molecular events including the spatiotemporal breakdown of the follicle wall. Recent work has shown that ovulation in Drosophila utilizes conserved cellular processes and molecular pathways as in mammals. Thus, Drosophila ovulation can serve as a good model to decipher the fundamental mechanisms of ovulation utilized across species. In past decades, several methods have been developed to study Drosophila ovulation, but all of them have drawbacks. This chapter offers a strategy and detailed protocols for performing and analyzing the necessary assays to evaluate the ovulation process in Drosophila.
排卵是一个关键的生殖过程，通过这个过程，一个成熟的卵母细胞从卵巢中释放出来以进行受精。这个过程需要多种细胞和分子事件的协调，包括卵泡壁的时空性破裂。最近的研究表明，果蝇的排卵过程与哺乳动物一样，利用了保守的细胞过程和分子途径。因此，果蝇排卵可作为一个很好的模型，用于解读跨物种排卵的基本机制。在过去几十年里，已经开发了几种方法来研究果蝇排卵，但所有这些方法都有缺点。本章提供了一种策略和详细的方案，用于进行和分析评估果蝇排卵过程所需的检测。
