Biose Ifechukwude Joachim, Bix Gregory Jaye
Clinical Neuroscience Research Center, Department of Neurosurgery, Tulane University School of Medicine, New Orleans, LA, USA.
Methods Mol Biol. 2023;2616:39-46. doi: 10.1007/978-1-0716-2926-0_5.
Bilateral carotid artery stenosis (BCAS) is a valid approach for modeling vascular dementia (VaD) in mice as it induces cerebral hypoperfusion and produces white matter degeneration and cognitive impairment. VaD is one of the major causes of cognitive impairment and currently has no approved therapy; hence its preclinical modeling is warranted for investigating potential therapeutic compounds. BCAS enables the characterization of brain pathology and associated cognitive phenotype of VaD. In this chapter, we describe the surgical method of inducing BCAS in mice, using titanium micro-coils, and we report cerebral blood flow changes before and after surgical induction as well as some histological findings in the corpus callosum of diabetic mice subjected to long-term BCAS.
双侧颈动脉狭窄（BCAS）是在小鼠中模拟血管性痴呆（VaD）的一种有效方法，因为它会导致脑灌注不足，并引起白质变性和认知障碍。VaD是认知障碍的主要原因之一，目前尚无获批的治疗方法；因此，其临床前模型对于研究潜在的治疗化合物是必要的。BCAS能够对VaD的脑病理学及相关认知表型进行表征。在本章中，我们描述了使用钛微线圈在小鼠中诱导BCAS的手术方法，并报告了手术诱导前后的脑血流变化，以及长期接受BCAS的糖尿病小鼠胼胝体的一些组织学发现。
