Department of Pharmacology, College of Medicine, University of Arizona, Tucson, AZ, USA.
Methods Mol Biol. 2023;2616:403-418. doi: 10.1007/978-1-0716-2926-0_28.
Drug permeability across the blood-brain barrier (BBB) is an important concept in the development of therapeutic strategies to treat neurological diseases such as ischemic stroke. These mechanisms can be evaluated in detail using cultured brain microvascular endothelial cells or intact animals subjected to experimental stroke. Here, we describe state-of-the-art approaches to study BBB transport of therapeutics using our in vitro and in vivo approaches. These methodologies allow for precise determination of transporter kinetic properties for currently marketed therapeutics or for new chemical entities that are under development as stroke drugs.
血脑屏障（BBB）的药物渗透性是开发治疗缺血性中风等神经疾病治疗策略的一个重要概念。可以使用培养的脑微血管内皮细胞或接受实验性中风的完整动物详细评估这些机制。在这里，我们描述了使用我们的体外和体内方法研究治疗药物 BBB 转运的最新方法。这些方法学允许精确确定目前市售治疗药物或正在开发为中风药物的新化学实体的转运体动力学特性。
