A Robust Ratiometric Fluorescent Sensor Based on Covalent Assembly of Dipeptides and Biomolecules for the High-Sensitive and Optosmart Detection of Pyrethroids.

In this study, two ultrashort dipeptides, diphenylalanine (FF) or C-terminal amidated diphenylalanine (DPA), were covalently self-assembled with genipin to obtain two well-defined supramolecular peptide nanoparticles for the detection of pyrethroids. DPA-genipin nanoparticles (PNPs) demonstrated excellent dual-emission fluorescence characteristics, tunable particle size, and robust photostability. Parallel to this, PNPs showed a ratiometric fluorescence response to λ-cyhalothrin (LC) with a distinct green-to-red color transition. The satisfactory self-calibration capability of the ratiometric system enabled PNPs to respond sensitively to LC in a wide range (5-800 μg/L) with a lower limit of detection of 0.034 μg/L. The introduction of a smartphone application made it easy to develop an intelligent evaluation platform based on PNPs, which had been proven to be applicable for on-site visualization of LC in agricultural products. The platform proposed here may be a new application of peptide self-assembly in sensing, with both important food safety implications and great economic value.