Center for Environment and Water Resources, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, PR China; Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, Changsha, 410083, PR China.
Center for Environment and Water Resources, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, PR China; Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, Changsha, 410083, PR China.
J Environ Manage. 2023 Apr 15;332:117340. doi: 10.1016/j.jenvman.2023.117340. Epub 2023 Jan 28.
To identify key species associated with pyrene degradation in Vallisneria natans (V.natans) rhizosphere sediment, this work investigated the temporal and spatial changes in the rhizosphere microbial community and the relationship between the changes and the pyrene degradation process through a three-compartment rhizome-box experiment under pyrene stress. The degradation kinetics of pyrene showed that the order of degradation rate was rhizosphere > near-rhizosphere > non-rhizosphere. The difference in the pyrene degradation behavior in the sediments corresponded to the change in the proportions of dominant phyla (Firmicutes and Proteobacteria) and genera (g_Massilia f_Comamonadaceae, g_Sphingomonas). The symbiosis networks and hierarchical clustering analysis indicated that the more important phyla related to the pyrene degradation in the rhizosphere was Proteobacteria, while g_Sphigomonas, f_Comamonadaceae, and especially g_Massilia were the core genera. Among them, f_Comamonadaceae was the genus most affected by rhizosphere effects. These findings strengthened our understanding of the PAHs-degradation microorganisms in V.natans rhizosphere and are of great significance for enhancing phytoremediation on PAHs-contaminated sediment.
为了鉴定与水蕴草（Vallisneria natans）根际沉积物中芘降解相关的关键物种，本研究通过芘胁迫下的三隔式根箱实验，调查了根际微生物群落的时空变化以及这些变化与芘降解过程之间的关系。芘的降解动力学表明，降解速率的顺序为根际>近根际>非根际。沉积物中芘降解行为的差异与优势门（Firmicutes 和 Proteobacteria）和属（g_Massilia f_Comamonadaceae、g_Sphingomonas）的比例变化相对应。共生网络和层次聚类分析表明，与根际芘降解相关的更重要的门是 Proteobacteria，而 g_Sphigomonas、f_Comamonadaceae，特别是 g_Massilia 是核心属。其中，f_Comamonadaceae 是受根际效应影响最大的属。这些发现加强了我们对水蕴草根际中多环芳烃降解微生物的理解，对增强受多环芳烃污染沉积物的植物修复具有重要意义。
