National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
Bioresour Technol. 2023 Mar;372:128689. doi: 10.1016/j.biortech.2023.128689. Epub 2023 Jan 27.
Efficient retention and enrichment of anammox bacteria (AnAOB) are essential for the application of municipal wastewater anammox. Herein, an innovative process for highly enriching AnAOB within suspended carrier was developed in a single-stage anaerobic/oxic/anoxic reactor with 5.5 % carrier filling ratio for real sewage. Addition of sludge fermentation products promoted stable maintenance of partial nitrification (nitrite accumulation rate > 90.0 %) and achieved efficient external sludge reduction (27.6-37.9 %). Continuous nitrite supply and carrier addition promoted AnAOB enrichment (2.4 × 10 gene copies/g dry sludge). Candidatus Brocadia was the predominant bacteria in carriers (18.6 %). The average effluents of total inorganic nitrogen (TIN) and NH-N were 1.9 and 0.8 mg/L with removal rates of 97.0 % and 98.7 %. In the anoxic stage, TIN removal rate reached 71.5 %, and the proportion of anammox to nitrogen removal accounted for 82.7 %. This study broadens the application of mainstream sewage anammox and the resource utilization of waste activated sludge.
高效保留和富集厌氧氨氧化菌（AnAOB）对于应用于城市污水的厌氧氨氧化工艺至关重要。本研究在 5.5%载体填充率的单级厌氧/好氧/缺氧反应器中开发了一种创新的悬浮载体中富集 AnAOB 的方法，用于实际污水。添加污泥发酵产物促进了稳定的部分硝化（亚硝酸盐积累率>90.0%），并实现了高效的外部污泥减量化（27.6-37.9%）。连续的亚硝酸盐供应和载体添加促进了 AnAOB 的富集（2.4×10基因拷贝/g 干污泥）。Candidatus Brocadia 是载体中主要的细菌（18.6%）。总无机氮（TIN）和 NH-N 的平均出水浓度分别为 1.9 和 0.8mg/L，去除率分别为 97.0%和 98.7%。在缺氧阶段，TIN 的去除率达到 71.5%，厌氧氨氧化对氮去除的比例占 82.7%。本研究拓宽了主流污水厌氧氨氧化的应用和废活性污泥的资源利用。
