Department of Chemical and Biological Engineering, University of Saskatchewan, 57 Campus Drive, Saskatoon, SK, S7N 5A9, Canada.
Division of Biomedical Engineering, University of Saskatchewan, 57 Campus Drive, Saskatoon, SK, S7N 5A9, Canada.
Sci Rep. 2023 Jan 30;13(1):1692. doi: 10.1038/s41598-023-27596-2.
Comprehensive understanding of protein adsorption phenomenon on membrane surface during hemodialysis (HD) is one of the key moments for development of hemocompatible HD membrane. Though many mechanisms and kinetics of protein adsorption on some surface have been studied, we are still far away from complete understanding and control of this process, which results in a series of biochemical reactions that causes severe complications with health and even the death among HD patients. The aim of this study is to conduct quantitative analysis of competitive adsorption tendency of human serum protein on polyether sulfone (PES) clinical dialysis membrane. In situ synchrotron radiation micro-computed tomography (SR-µCT) imaging available at the Canadian Light Source (CLS) was conducted to assess human serum proteinbinding and undertake the corresponding quantitative analysis.The competitive adsorption of Human protein albumin (HSA), fibrinogen (FB) and transferrin (TRF) were tested from single and multiple protein solution. Furthermore, in-vitro human serum protein adsorption on clinical dialyzers was investigated using UV-Visible to confirm the competitive adsorption tendency. Results showed that when proteins were adsorbed from their mixture, FB content (among proteins) in the adsorbed layer increased from 3.6% mass (content in the initial solution) to 18% mass and 12%, in case of in situ quantitative and invitro analysis, respectively. The increase in FB content was accompanied by the decrease in the HSA content, while TRF remained on approximately on the same level for both cases. Overall, the percentage of HSA adsorption ratio onto the HD membrane has dropped approximately 10 times when HSA was adsorbed in competition with other proteins, compared to the adsorption from single HSA solution. The substitution of HSA with FB was especially noticeable when HSA adsorption from its single solution was compared with the case of the protein mixture. Moreover, SR-µCT has revealed that FB when adsorbed from a protein mixture solution is located predominately in the middle of the membrane, whereas the peak of the distribution is shifted to membrane bottom layers when adsorption from FB single solution takes place. Results showed that HSA FB and TRF adsorption behavior observations are similar on both in-situ small scale and clinical dialyzer of the PES membrane.
全面了解血液透析（HD）过程中蛋白质在膜表面的吸附现象是开发血液相容性 HD 膜的关键之一。尽管已经研究了许多蛋白质在某些表面上的吸附机制和动力学，但我们仍然远未完全理解和控制这一过程，这导致了一系列生化反应，从而给 HD 患者的健康甚至生命带来严重并发症。本研究旨在对聚醚砜（PES）临床透析膜上人血清蛋白的竞争吸附倾向进行定量分析。加拿大光源（CLS）的同步辐射微计算机断层扫描（SR-µCT）成像可用于评估人血清蛋白结合并进行相应的定量分析。从单一和多种蛋白质溶液中测试了人白蛋白（HSA）、纤维蛋白原（FB）和转铁蛋白（TRF）的竞争吸附。此外，使用紫外-可见分光光度法研究了临床透析器上的体外人血清蛋白吸附，以确认竞争吸附倾向。结果表明，当从混合物中吸附蛋白质时，吸附层中 FB 的含量（蛋白质中）从初始溶液中的 3.6%质量增加到 18%质量和 12%质量，分别为原位定量和体外分析。FB 含量的增加伴随着 HSA 含量的减少，而 TRF 在两种情况下的含量基本相同。总体而言，与从单一 HSA 溶液中吸附相比，当 HSA 与其他蛋白质竞争吸附时，HSA 吸附到 HD 膜上的比例下降了约 10 倍。当将 HSA 从其单一溶液中的吸附与蛋白质混合物的情况进行比较时，用 FB 替代 HSA 尤其明显。此外，SR-µCT 表明，当从蛋白质混合物溶液中吸附 FB 时，其主要位于膜的中间，而当从 FB 单一溶液中吸附时，分布的峰值移至膜的底层。结果表明，在 PES 膜的原位小尺度和临床透析器上，观察到 HSA、FB 和 TRF 的吸附行为相似。
