Yousif Hussin Alimam Hoyam, Abdelateif Hussein Waleed, Ibrahim Sabah, Abdelgani Sara, Alharthi Nahed, Bashier Eltayeb Lienda, Abdelgadir Elmahdi Salih, Abobakr Abdrabo AbdElkarim
Department of Clinical Chemistry, Faculty of Medical Laboratory Science, Al-Neelain University, Khartoum Sudan.
Department of Immunology, Faculty of Medical Laboratory Science, Al-Neelain University, Khartoum Sudan.
Rep Biochem Mol Biol. 2022 Oct;11(3):421-429. doi: 10.52547/rbmb.11.3.421.
Diabetes-related vascular complications linked to increase in the expression of VEGF and its receptors. It helps to accelerate tissue damage inflicted by hyperglycemia, which is potential risk for diabetic complications. The study aimed to assess VEGF genetic polymorphism and its correlation with glucose and HbA1C level among Sudanese patients with diabetic retinopathy and nephropathy.
A case-control study was conducted among a total of 252 subjects and divided into four groups of 63 subjects each. Glucose and HBA1c were measured then the VEGF gene was amplified using polymerase chain reaction. The data were analyzed using SPSS.
The HBA1c, and blood glucose levels had significantly (P value≤0.00001) highest mean in the DR group, DN group followed by DM. There is a non-significant correlation between VEGF Genotypes and HbA1c, and blood glucose levels (P value≤0.102, 0.173) Patients with GC genotypes will be 74.6%, and 54% higher at risk to develop DR, and DN respectively and 40 % lower at risk to develop DM than those without GC genotype. While patients with CC genotypes will be 22.2% higher at risk of developing DM and 9.5%, 12.2% higher at risk of developing DR and DN respectively.
The VEGF +405G/C gene polymorphism is linked to diabetic retinopathy, and diabetic nephropathy in type 2 Sudanese diabetics, and the presence of the GC genotypes and G allele is a significant predictor for retinopathy. There is no significant relation between HbA1C serum levels, blood glucose, and the VEGF +405G/C gene polymorphism.
糖尿病相关血管并发症与血管内皮生长因子（VEGF）及其受体表达增加有关。它有助于加速高血糖造成的组织损伤，这是糖尿病并发症的潜在风险。本研究旨在评估苏丹糖尿病视网膜病变和肾病患者中VEGF基因多态性及其与血糖和糖化血红蛋白（HbA1C）水平的相关性。
对总共252名受试者进行了病例对照研究，分为四组，每组63名受试者。测量血糖和糖化血红蛋白，然后使用聚合酶链反应扩增VEGF基因。使用SPSS分析数据。
糖化血红蛋白和血糖水平在糖尿病视网膜病变（DR）组中平均显著最高（P值≤0.00001），糖尿病肾病（DN）组次之，然后是糖尿病（DM）组。VEGF基因型与糖化血红蛋白和血糖水平之间无显著相关性（P值≤0.102、0.173）。与没有GC基因型的患者相比，具有GC基因型的患者发生DR和DN的风险分别高74.6%和54%，发生DM的风险低40%。而具有CC基因型的患者发生DM的风险高22.2%，发生DR和DN的风险分别高9.5%和12.2%。
VEGF +405G/C基因多态性与2型苏丹糖尿病患者的糖尿病视网膜病变和糖尿病肾病有关，GC基因型和G等位基因的存在是视网膜病变的重要预测指标。糖化血红蛋白血清水平、血糖与VEGF +405G/C基因多态性之间无显著关系。
