College of Engineering and Technology, Northeast Forestry University, Harbin 150040, PR China.
College of Engineering and Technology, Northeast Forestry University, Harbin 150040, PR China.
Int J Biol Macromol. 2023 Mar 31;232:123464. doi: 10.1016/j.ijbiomac.2023.123464. Epub 2023 Jan 30.
In order to overcome this challenge of poor stability of natural anthocyanins in intelligent packaging materials, roselle anthocyanin (RA) was first modified by acetic acid, and then a double-layer smart indication antimicrobial film was developed using modified roselle anthocyanin (MRA)-gellan gum (GG) as the inner layer and sodium carboxymethyl cellulose (CMC)-starch (ST)-Nisin as the outer layer. UV spectra revealed that acetic acid was successfully grafted onto RA, which dramatically improved their thermal stability, antioxidant capabilities, photostability, and pH stability. The bilayer films were successfully prepared, as revealed by scanning electron microscopy, Fourier-transform infrared spectroscopy, and X-ray diffraction measurements. In comparison to GG-MRA and CMC-ST-Nisin films, the water content, water solubility, mechanical characteristics, water vapor barrier, oxygen barrier, and hydrophobicity of GG-MRA@CMC-ST-Nisin films were significantly enhanced. The presence of the outer layer films significantly enhanced the UV-vis light barrier, opacity, antioxidant and antibacterial properties of the inner layer films. When the films were applied to chicken breast, it was found that the indicator films not only monitored the freshness of the chicken in real-time but also that the GG-MRA film and the double-layer film were effective in extending the shelf life of the chicken by 1 and 2 days, respectively, compared to the control group.
为了克服智能包装材料中天然花色苷稳定性差的这一挑战，首先用乙酸对玫瑰茄花色苷（RA）进行修饰，然后用修饰后的玫瑰茄花色苷（MRA）-结冷胶（GG）作为内层，羧甲基纤维素钠（CMC）-淀粉（ST）-乳链菌肽（Nisin）作为外层，开发出双层智能指示型抗菌膜。紫外光谱表明，乙酸成功地接枝到 RA 上，这极大地提高了它们的热稳定性、抗氧化能力、光稳定性和 pH 值稳定性。扫描电子显微镜、傅里叶变换红外光谱和 X 射线衍射测量表明成功制备了双层膜。与 GG-MRA 和 CMC-ST-Nisin 膜相比，GG-MRA@CMC-ST-Nisin 膜的水分含量、水溶性、力学性能、水蒸气阻隔性、氧气阻隔性和疏水性均显著提高。外层膜的存在显著提高了内层膜的紫外可见光阻隔性、不透明度、抗氧化和抗菌性能。当将这些薄膜应用于鸡胸肉时，发现指示薄膜不仅可以实时监测鸡肉的新鲜度，而且与对照组相比，GG-MRA 薄膜和双层薄膜分别将鸡肉的保质期延长了 1 天和 2 天。
