Wu Hao, Tong Youmin, Wang Cuiming, Yu Yashuai, Chen Mingyue, Wang Yulong, Li Xiaojuan, Huang Bo
Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei 230036, China; School of Life Sciences, Anhui Medical University, Hefei 230032, China.
Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei 230036, China.
J Invertebr Pathol. 2023 Mar;197:107892. doi: 10.1016/j.jip.2023.107892. Epub 2023 Jan 30.
Pigments of conidia play a crucial role in fungal defense against environmental stressors such as UV radiation. The molecular basis of conidial pigmentation has been studied in the entomopathogenic fungus Metarhizium robertsii, while limited information been reported on function mechanisms transcription factors governing conidial pigmentation. Here, we identified transcription factor MrAbaA binding to the promoter regions of both MrPks1 and MrMlac1 in M. robertsii using yeast one-hybrid technology. Chromatin immunoprecipitation quantitative PCR assays further confirmed the interaction. Furthermore, overexpression of MrAbaA in M. robertsii resulted in increased conidial pigment accumulation and enhanced tolerances to UV stress by upregulated the MrPks1 and MrMlac1 expression. Taken together, MrAbaA affects conidial pigmentation by interacting with the promoter regions of both MrPks1 and MrMlac1 in M. robertsii. This work advances the understanding of the regulation mechanism for conidial pigmentation in entomopathogenic fungi.
分生孢子色素在真菌抵御紫外线辐射等环境应激源中起着关键作用。人们已对昆虫病原真菌罗伯茨绿僵菌分生孢子色素沉着的分子基础进行了研究，但关于调控分生孢子色素沉着的转录因子功能机制的报道却很有限。在此，我们利用酵母单杂交技术，鉴定出罗伯茨绿僵菌中与MrPks1和MrMlac1启动子区域结合的转录因子MrAbaA。染色质免疫沉淀定量PCR分析进一步证实了这种相互作用。此外，在罗伯茨绿僵菌中过表达MrAbaA会导致分生孢子色素积累增加，并通过上调MrPks1和MrMlac1的表达增强对紫外线胁迫的耐受性。综上所述，MrAbaA通过与罗伯茨绿僵菌中MrPks1和MrMlac1的启动子区域相互作用来影响分生孢子色素沉着。这项工作推进了对昆虫病原真菌分生孢子色素沉着调控机制的理解。
