Zhang Z X, Xu S H, Qian L J
Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou.
Sci Sin B. 1987 May;30(5):522-32.
A human rectal poorly-differentiated adenocarcinoma cell line HR-8348 is established from the surgically removed tumor mass of a male adult patient. The cell doubling time is 36.05 h. The mitotic index is 44.5%. The cells are all of epithelial type and mostly of polygonal shape. Electron microscopic examination shows malignant nuclei with enlarged nucleoli; there are abundant microvilli and some secretory granules. The plating efficiency in soft agar is 12.9%. In PHA (1 microgram/ml), the cells show obvious agglutination. Chromosomal analysis reveals a mode of 70 chromosomes per cell, with a marker chromosome. CEA in cells is detected positively both qualitatively and quantitatively. Hetero-transplantation of the cells to nude mice shows that the tumor mass has the same morphology as the original mass from the patient, with Alcian blue positive material in the cytoplasm. Repeated detection for mycoplasma contamination shows negative.
人直肠低分化腺癌细胞系HR - 8348源自一名成年男性患者手术切除的肿瘤块。细胞倍增时间为36.05小时。有丝分裂指数为44.5%。细胞均为上皮型，大多呈多边形。电子显微镜检查显示细胞核恶性，核仁增大；有丰富的微绒毛和一些分泌颗粒。软琼脂平板接种效率为12.9%。在PHA（1微克/毫升）中，细胞显示明显凝集。染色体分析显示每个细胞有70条染色体的众数，并伴有一条标记染色体。细胞中癌胚抗原（CEA）定性和定量检测均呈阳性。将细胞异种移植到裸鼠体内显示，肿瘤块与患者的原始肿瘤块形态相同，细胞质中有阿尔辛蓝阳性物质。支原体污染的反复检测显示为阴性。
