肝癌缺失基因1通过抑制Rho相关蛋白激酶途径抑制前列腺癌细胞的生长。
Deleted in liver cancer 1 suppresses the growth of prostate cancer cells through inhibiting Rho-associated protein kinase pathway.
作者信息
Gong Hua, Chen Kang, Zhou Lan, Jin Yongchao, Chen Weihua
机构信息
Department of Urology, Zhoupu Hospital, Medicine and Health Sciences, Shanghai University, Shanghai, China.
Department of Urology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, China.
出版信息
Asian J Urol. 2023 Jan;10(1):50-57. doi: 10.1016/j.ajur.2021.12.007. Epub 2022 Jan 12.
OBJECTIVE
Deleted in liver cancer 1 (DLC1) is a GTPase-activating protein that is reported as a suppressor in certain human cancers. However, the detailed biological function of DLC1 is still unclear in human prostate cancer (PCa). In the present study, we aimed to explore the function of DLC1 in PCa cells.
METHODS
Silencing and overexpression of DLC1 were induced in an androgen-sensitive PCa cell line (LNCaP) using RNA interference and lentiviral vector transduction. The Cell Counting Kit-8 assay was performed to determine cell proliferation. The cell cycle was examined by performing a propidium iodide staining assay.
RESULTS
Our results indicated that DLC1 overexpression markedly suppressed the proliferation and cell cycle progression of LNCaP cells. Moreover, DLC1 expression was negatively correlated with Rho-associated protein kinase (ROCK) expression in LNCaP cells. Importantly, this study showed that the ROCK inhibitor Y27632 restored the function of DLC1 in LNCaP cells and reduced the tumorigenicity of LNCaP cells .
CONCLUSION
Our results indicated that DLC1 overexpression markedly suppressed the proliferation and cell cycle progression of PCa cells and negatively correlated with ROCK expression in PCa cells and tissue.
目的
肝癌缺失基因1（DLC1）是一种GTP酶激活蛋白，在某些人类癌症中被报道为一种抑癌基因。然而，DLC1在人类前列腺癌（PCa）中的详细生物学功能仍不清楚。在本研究中，我们旨在探讨DLC1在PCa细胞中的功能。
方法
使用RNA干扰和慢病毒载体转导在雄激素敏感的PCa细胞系（LNCaP）中诱导DLC1的沉默和过表达。采用细胞计数试剂盒-8法检测细胞增殖情况。通过碘化丙啶染色法检测细胞周期。
结果
我们的结果表明，DLC1过表达显著抑制LNCaP细胞的增殖和细胞周期进程。此外，LNCaP细胞中DLC1的表达与Rho相关蛋白激酶（ROCK）的表达呈负相关。重要的是，本研究表明ROCK抑制剂Y27632恢复了DLC1在LNCaP细胞中的功能，并降低了LNCaP细胞的致瘤性。
结论
我们的结果表明，DLC1过表达显著抑制PCa细胞的增殖和细胞周期进程，且与PCa细胞及组织中ROCK的表达呈负相关。
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