Biopesticides laboratory, Centre of Biotechnology of Sfax, Sfax University, PO Box 1177, 3018 Sfax, Tunisia.
Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue Taher Hadded (B.P 74), 5000 Monastir, Tunisia.
J Appl Microbiol. 2023 Mar 1;134(3). doi: 10.1093/jambio/lxad018.
This study aimed to improve the screening strategy for the selection of biocontrol agents with high biocontrol efficacy against fire blight disease.
A two-step screening procedure consisting of in-vitro laboratory tests and an ex-vivo test system using detached pear leaves was applied to 43 Bacillus strains originated from the rhizosphere and the aerial parts of apple and pear plants. The grouping of the studied strains and the tested traits based on the principal component analysis and the two-way hierarchical cluster analysis showed that siderophore production and biofilm formation are the most desirable traits in a Bacillus biocontrol agent to control fire blight disease and that rhizospheric originating strains are the most effective.
In contrast to the previous screening strategies that are often insufficient to select the most suitable microorganisms, this study reported an improved strategy based on the microbial competition traits to select potential Bacillus biocontrol agents with high biocontrol efficacy against fire blight disease.
本研究旨在改进生防制剂的筛选策略，以选择对火疫病具有高效防治效果的生防制剂。
采用两步筛选程序，包括体外实验室测试和使用离体梨叶片的体外测试系统，对 43 株源于苹果树和梨树根际和地上部分的芽孢杆菌进行了筛选。基于主成分分析和双向层次聚类分析对研究菌株和测试特性进行分组，结果表明，铁载体生产和生物膜形成是芽孢杆菌生防制剂控制火疫病最理想的特性，而根际来源的菌株则是最有效的。
与以往筛选策略往往不足以选择最适宜的微生物相比，本研究报告了一种基于微生物竞争特性的改进策略，以选择对火疫病具有高效防治效果的潜在芽孢杆菌生防制剂。
