无内毒素 Stx2B-C-CPE 疫苗及其优化的佐剂方案预防食物中毒。
Endotoxin-Free Stx2B-C-CPE Vaccine and Its Optimized Adjuvant Regimen for Preventing Food Poisoning.
机构信息
Laboratory of Vaccine Materials, Center for Vaccine and Adjuvant Research, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), 567-0085 Ibaraki, Osaka, Japan.
Laboratory of Gut Environmental System, Collaborative Research Center for Health and Medicine, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), 567-0085 Ibaraki, Osaka, Japan.
出版信息
Front Biosci (Landmark Ed). 2023 Jan 17;28(1):15. doi: 10.31083/j.fbl2801015.
BACKGROUND
and Shiga toxin (Stx)-producing (STEC) are common causes of food poisoning. We previously demonstrated the efficacy of Stx2B-C-CPE, a fusion protein of the C-terminal region of enterotoxin (C-CPE) and Shiga toxin 2 B subunit (Stx2B), as a bivalent vaccine against and STEC infections.
METHODS
Here, we applied an expression system and Triton X-114 phase separation to prepare tag- and endotoxin-free Stx2B-C-CPE for use in vaccine formulations.
RESULTS
As we anticipated, endotoxin removal from the purified antigen reduced both Stx2B- and C-CPE-specific IgG antibody responses in subcutaneously immunized mice, suggesting that endotoxin contamination influences the immunological assessment of Stx2B-C-CPE. However, the combined use of aluminum and lipid A adjuvants improved IgG antibody responses to the injected antigen, thus indicating the suitability of purified Stx2B-C-CPE for vaccine formulation.
CONCLUSIONS
Our current findings provide important knowledge regarding the design of an effective commercial Stx2B-C-CPE vaccine.
背景
产志贺毒素（Stx）-大肠埃希氏菌（STEC）是食物中毒的常见原因。我们之前证明了 Shiga 毒素 2B-C-CPE（一种肠毒素 C 端区域（C-CPE）和 Shiga 毒素 2B 亚基（Stx2B）融合蛋白）作为针对 和 STEC 感染的二价疫苗的功效。
方法
在这里，我们应用了 表达系统和 Triton X-114 相分离来制备标签和内毒素免费的 Stx2B-C-CPE，用于疫苗配方。
结果
正如我们所预期的，从纯化抗原中去除内毒素降低了皮下免疫小鼠的 Stx2B 和 C-CPE 特异性 IgG 抗体反应，表明内毒素污染会影响 Stx2B-C-CPE 的免疫学评估。然而，铝和 脂质 A 佐剂的联合使用提高了对注射抗原的 IgG 抗体反应，因此表明纯化的 Stx2B-C-CPE 适合疫苗配方。
结论
我们目前的研究结果为设计有效的商业 Stx2B-C-CPE 疫苗提供了重要的知识。
Zotero 插件