一种具有生物相容性和选择性的“双钥匙和锁”比率荧光探针，用于成像相邻二硫蛋白。
A "dual-key-and-lock" ratiometric fluorescent probe with biocompatibility and selectivity for imaging vicinal dithiol proteins.
机构信息
Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.
出版信息
Chem Commun (Camb). 2023 Feb 9;59(13):1785-1788. doi: 10.1039/d2cc06422g.
TMR-TPE, a ratiometric fluorescent probe, was reported for the imaging of vicinal dithiol proteins (VDPs) in living cells. Profiting from the "dual-key-and-lock" design, TMR-TPE solves the toxicity problem of VDP probes (98% cell viability at 50 μM) and avoids the interference of small thiols (up to 10 mM GSH). The change of VDPs during drug-induced liver injury was monitored for the first time using TMR-TPE.
TMR-TPE 是一种比率型荧光探针，用于活细胞中毗邻二硫键蛋白（VDP）的成像。得益于“双钥匙-锁”设计，TMR-TPE 解决了 VDP 探针的毒性问题（在 50μM 时细胞存活率为 98%），并避免了小分子硫醇（高达 10mM GSH）的干扰。首次使用 TMR-TPE 监测了药物性肝损伤过程中 VDP 的变化。
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