Didenko Maria M, Yastrub Tatyana O, Hrygorieva Kateryna V, Dontsova Dariya O
STATE INSTITUTION "KUNDIIEV INSTITUTE OF OCCUPATIONAL HEALTH OF THE NATIONAL ACADEMY OF MEDICAL SCIENCES OF UKRAINE", KYIV, UKRAINE.
Wiad Lek. 2022;75(12):2987-2993. doi: 10.36740/WLek202212116.
The aim: To determine the dose dependence of the subchronic effect of acetamiprid on the body of rats based on the data of morphological studies of internal organs.
Materials and methods: The experiment was performed on Wistar Han rats, which were orally administered acetamiprid in doses of 6, 12 and 60 mg/kg for 13 weeks. During the experiment, clinical studies were carried out, the general condition of the animals, body weight were assessed. After necropsy, the absolute and relative weight of internal organs was determined, and morphological studies of the brain, liver, kidneys, and spleen were performed with using an Olympus BX 54 light microscope and an Olympus C-5050 ZOOM camera with software Olympus DP-Soft. The research results were subjected to statistical processing using the Microsoft Excel 2010 computer program package.
Results: The most pronounced manifestations of the toxic effect of acetamiprid were observed at a dose of 60 mg/kg, which indicated its hepatotoxic and nephrotoxic effects, as well as neurotoxic effects with signs of irreversible neurocyte damage.
Conclusions: Morphological studies showed a dose-dependent nature and degree of expressiveness of the toxic effect of acetamiprid. According to the totality and nature of the changes revealed in the conditions of the conducted subchronic experiment on rats, no observed adverse effect level (NOAEL) was determined at the level of 12 mg/kg, no observed effect level (NOEL) - 6 mg/kg.
基于对大鼠内脏器官的形态学研究数据，确定啶虫脒对大鼠机体亚慢性效应的剂量依赖性。
材料与方法：实验选用Wistar Han大鼠，以6、12和60 mg/kg的剂量口服给予啶虫脒，持续13周。实验期间进行临床研究，评估动物的一般状况和体重。剖检后，测定内脏器官的绝对重量和相对重量，并使用Olympus BX 54光学显微镜和配备Olympus DP-Soft软件的Olympus C-5050 ZOOM相机对脑、肝、肾和脾进行形态学研究。研究结果使用Microsoft Excel 2010计算机程序包进行统计处理。
结果：在60 mg/kg剂量下观察到啶虫脒毒性作用的最明显表现，表明其具有肝毒性和肾毒性作用，以及伴有不可逆神经细胞损伤迹象的神经毒性作用。
结论：形态学研究表明啶虫脒毒性作用具有剂量依赖性和表达程度。根据在对大鼠进行的亚慢性实验条件下所揭示变化的总体情况和性质，在12 mg/kg水平未确定未观察到有害作用水平（NOAEL），在6 mg/kg水平未确定未观察到作用水平（NOEL）。
