College of Chemistry and Chemical Engineering, Donghua University, Shanghai, 201620, China.
Zhejiang Engineering Research Center for Tissue Repair Materials, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, 325000, China.
J Mater Chem B. 2023 Jun 21;11(24):5469-5482. doi: 10.1039/d2tb02614g.
Calcium phosphate (CaP) bioceramics are broadly employed for bone regeneration due to their excellent biocompatibility and osteoconductivity. However, they are not capable of repairing healing-impaired bone defects such as defects with conditions of ischemia or infection due to restricted bioactivities. In this study, we synthesized single-phased strontium-zinc-phosphate (SZP, SrZn(PO)) bioceramics a solution combustion method and further fabricated SZP scaffolds using a three-dimensional (3D) printing technique. Compared to 3D printed β-tricalcium phosphate (β-TCP) scaffolds, the 3D printed SZP scaffolds presented comparable porosity, compressive strength, and Young's modulus, but increased ability of osteogenesis, angiogenesis, immunomodulation and anti-bacterial activity. Specifically, 3D printed SZP scaffolds not only led to significantly higher osteogenic differentiation of MC3T3-E1 cells and pro-angiogenesis of human umbilical vein endothelial cells (HUVECs) directly or through macrophage-mediated immunomodulation, but also inhibited the growth of () and (). The study of the rat cranial bone defect model further confirmed better vascularized bone regeneration in 3D-printed SZP scaffolds. These findings indicate that the proposed 3D-printed SZP scaffolds might be a versatile candidate for bone tissue engineering.
钙磷酸盐（CaP）生物陶瓷由于其优异的生物相容性和骨诱导性而被广泛用于骨再生。然而，由于其生物活性有限，它们无法修复愈合受损的骨缺损，如缺血或感染条件下的骨缺损。在本研究中，我们采用溶液燃烧法合成了单相锶锌磷酸盐（SZP，SrZn（PO ））生物陶瓷，并进一步采用三维（3D）打印技术制备了 SZP 支架。与 3D 打印的β-磷酸三钙（β-TCP）支架相比，3D 打印的 SZP 支架具有相当的孔隙率、抗压强度和杨氏模量，但成骨、血管生成、免疫调节和抗菌活性得到了增强。具体而言，3D 打印的 SZP 支架不仅直接或通过巨噬细胞介导的免疫调节导致 MC3T3-E1 细胞的成骨分化和人脐静脉内皮细胞（HUVECs）的促血管生成显著增加，而且还抑制了（）和（）的生长。大鼠颅骨缺损模型的研究进一步证实了 3D 打印 SZP 支架中更好的血管化骨再生。这些发现表明，所提出的 3D 打印 SZP 支架可能是一种多功能的骨组织工程候选材料。
