Shokri Abbas, Moradhaseli Hanyeh, Fekrazad Reza, Jazaeri Mina, Farhadian Maryam
Dental Implants Research Center, Department of Oral and Maxillofacial Radiology, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran.
Dentist, Hamadan University of Medical Sciences, Hamadan, Iran.
Lasers Med Sci. 2023 Feb 1;38(1):59. doi: 10.1007/s10103-023-03714-4.
Osteoporosis is associated with severe pain, bone deformity, fracture, and bone loss. It is important to find strategies to prevent bone resorption and treat osteoporosis. This study sought to assess the effect of photobiomodulation therapy (PBMT) with different wavelengths on bone mineral density (BMD) in osteoporotic rats. This animal study evaluated 63 adult female rats. The rats underwent ovariectomy to induce osteoporosis. Ovariectomized rats were randomly divided into 9 groups of control (OC), treatment with zoledronic acid alone (0.02 mg/kg), and treatment with 660 nm, 810 nm, and 940 nm PBMT alone (3 times a week for 6 weeks, energy density of 4 J/cm), and combined with zoledronic acid. The healthy control group (HC) only underwent sham surgery. The rats underwent cone-beam computed tomography (CBCT) 52 days after the first treatment session to measure their BMD according to the gray value (GV) of images. To assess the biomechanical properties of bone, the resected bones were subjected to 3-point bending test (3-PBT). The experimental groups had significant differences with the OC group regarding radiographic and biomechanical properties of bone (P < 0.05), indicating a healing course. No significant difference was noted between the experimental groups treated with different laser wavelengths and those treated with zoledronic acid (P > 0.05). In the condition of this study, it was found that PBMT at a constant energy density of 4 J/cm with 660-, 810-, and 940-nm wavelengths is effective for enhancement of bone mineral density and biomechanical properties. No significant difference was noted between different wavelengths of diode laser regarding radiographic and biomechanical properties of bone.
骨质疏松症与严重疼痛、骨骼畸形、骨折和骨质流失有关。找到预防骨质吸收和治疗骨质疏松症的策略很重要。本研究旨在评估不同波长的光生物调节疗法（PBMT）对骨质疏松大鼠骨密度（BMD）的影响。这项动物研究评估了63只成年雌性大鼠。对大鼠进行卵巢切除术以诱导骨质疏松症。将去卵巢大鼠随机分为9组：对照组（OC）、单独使用唑来膦酸治疗（0.02mg/kg）、单独使用660nm、810nm和940nm PBMT治疗（每周3次，共6周，能量密度为4J/cm²）以及联合唑来膦酸治疗。健康对照组（HC）仅接受假手术。在第一次治疗后52天，对大鼠进行锥形束计算机断层扫描（CBCT），根据图像的灰度值（GV）测量其骨密度。为了评估骨骼的生物力学特性，对切除的骨骼进行三点弯曲试验（3-PBT）。实验组在骨的影像学和生物力学特性方面与OC组有显著差异（P<0.05），表明有愈合过程。不同激光波长治疗的实验组与唑来膦酸治疗的实验组之间未观察到显著差异（P>0.05）。在本研究条件下，发现波长为660nm、810nm和940nm、能量密度恒定为4J/cm²的PBMT对提高骨密度和生物力学特性有效。不同波长的二极管激光在骨的影像学和生物力学特性方面未观察到显著差异。
