植物乳杆菌 P101 通过调节 Nrf2/HO-1 通路减轻小鼠酒精性肝损伤。

Lactiplantibacillus plantarum P101 alleviates alcoholic liver injury by modulating the Nrf2/HO-1 pathway in mice.

机构信息

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.

出版信息

J Appl Microbiol. 2023 Jan 23;134(1). doi: 10.1093/jambio/lxac032.

DOI:10.1093/jambio/lxac032

PMID:36724245

Abstract

AIMS

The occurrence of alcoholic liver injury is related to the oxidative stress. Bacteria for alleviating alcoholic related liver injury have received widespread attention. Study aims to investigate the alleviated efficacy of Lactiplantibacillus plantarum (L. plantarum) P101 on alcohol-induced liver injury and its potential mechanism.

METHODS AND RESULTS

The model of alcoholic liver injury was obtained according to the NIAAA method and the mice were treated with L. plantarum P101 (108 CFU.mice-1). Results showed that treatment of L. plantarum P101 could significantly improve liver function and antioxidant capacity. Furthermore, L. plantarum P101 significantly up-regulated Nuclear factor erythroid 2-related factor (Nrf2) and its target molecule, Hemeoxygenase 1 (HO-1), by promoting nuclear translocation of Nrf2. Moreover, inflammatory factors and pro-apoptotic protein (Caspase3) levels were significantly decreased in mice treated with L. plantarum P101.

CONCLUSIONS

This study confirmed that the beneficial effect of L. plantarum P101 supplement was achieved via regulating Nrf2/HO-1 antioxidant pathway, and alleviated alcoholic liver injury.

摘要

目的

酒精性肝损伤的发生与氧化应激有关。具有缓解酒精相关肝损伤作用的细菌受到广泛关注。本研究旨在探讨植物乳杆菌 P101 对酒精性肝损伤的缓解作用及其潜在机制。

方法和结果

根据 NIAAA 方法建立酒精性肝损伤模型，并用植物乳杆菌 P101（108 CFU·小鼠-1）处理小鼠。结果表明，植物乳杆菌 P101 处理可显著改善肝功能和抗氧化能力。此外，植物乳杆菌 P101 通过促进 Nrf2 的核易位，显著上调核因子红细胞 2 相关因子（Nrf2）及其靶分子血红素加氧酶 1（HO-1）。而且，用植物乳杆菌 P101 处理的小鼠中炎症因子和促凋亡蛋白（Caspase3）水平显著降低。