Quantum Biophotonic Lab, Key Laboratory of Optical Technology and Instrument for Medicine, Ministry of Education, School of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001, China.
ACS Appl Mater Interfaces. 2023 Feb 15;15(6):8224-8231. doi: 10.1021/acsami.2c22413. Epub 2023 Feb 1.
The carbonyl groups of deoxyribonucleotide can resonantly couple with 53 THz middle infrared, which can highly transmit water without ionization-based damage to DNA molecules. Herein, we predict that vibrational coupling with THz irradiation could lower down the hybridization landscape of nucleic acids and thus affect DNA replication. Using polymerase chain reaction (PCR) as a measure, we found that THz shining can reduce the denature temperature of DNA duplexes by about 3 °C, which allows one to conduct PCR at lower temperature, facilitating long-time amplification reaction without losing enzymatic fidelity, i.e., normal PCR should be carried out at denaturing temperature ∼4 °C higher than the melting temperature (), but THz-PCR only requires temperature ∼1 °C higher than due to the nonthermal effect of THz shining. Moreover, the melting time can also be shortened to 1/5 due to the enhanced vibration coupling with 53 THz irradiation. We proposed THz-PCR as an innovated DNA amplification technique with ultrahigh specificity and sensitivity and also successfully demonstrated its advantages in forensic detections.
脱氧核苷酸的羰基可以与 53THz 中红外发生共振耦合，中红外可以在不使 DNA 分子发生基于离子化的损伤的情况下高度透过水。在此，我们预测振动耦合与太赫兹辐照会降低核酸的杂交势垒，从而影响 DNA 复制。使用聚合酶链反应（PCR）作为衡量标准，我们发现太赫兹照射可以将 DNA 双链体的变性温度降低约 3°C，这使得人们可以在较低的温度下进行 PCR，有利于长时间的扩增反应而不会失去酶的保真度，即，标准 PCR 应在比熔点（）高约 4°C 的变性温度下进行，但由于太赫兹照射的非热效应，太赫兹-PCR 仅需要比高约 1°C 的温度即可。此外，由于与 53THz 辐照的振动耦合增强，熔化时间也可以缩短至 1/5。我们提出太赫兹-PCR 作为一种具有超高特异性和灵敏度的新型 DNA 扩增技术，并成功地在法医检测中证明了其优势。
