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利用海草海菖蒲(R.Br.)Asch. & 马格努斯进行银纳米粒子的绿色合成、表征以及抗癌、抗氧化和抗血糖指数的评估。

Green synthesis of silver nanoparticles using seagrass Cymodocea serrulata (R.Br.) Asch. & Magnus, characterization, and evaluation of anticancer, antioxidant, and antiglycemic index.

机构信息

Department of Physiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical & Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India.

Marine Biomedical Research Lab & Environmental Toxicology Unit, Cellular and Molecular Research Centre, Department of Prosthodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical & Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India.

出版信息

Biotechnol Appl Biochem. 2023 Jun;70(3):1346-1356. doi: 10.1002/bab.2444. Epub 2023 Feb 11.

Abstract

In the present study, the green synthesis of silver nanoparticles (AgNPs) using ethanol extract of Cymodocea serrulata and biological activity were investigated by UV-visible spectrophotometer, Fourier-transform infrared spectroscopy (FTIR), x-ray powder diffraction (XRD), and scanning electron microscopy. The results show that nanoparticles synthesized were confirmed by color change from green to dark brown. The XRD analysis confirmed that the AgNPs were crystalline and found that their UV maximum specific absorbance was between 200 and 400 nm, and their field emission scanning electron microscopy size was between 60 and 69 nm. FTIR studies identified different functional groups involved in the potential capping of AgNPs. The antidiabetic activity of the AgNPs was tested by the inhibition of carbohydrate digestive enzymes (a-glucosidase and amylase). In addition, it has exhibited potential anticancer activity against breast cancer cells (MDF7). Hence, the present result warrants ecofriendly and efficient method in the synthesis of AgNPs, which can act as an alternative biomaterial for biomedical applications.

摘要

在本研究中,通过紫外可见分光光度计、傅里叶变换红外光谱(FTIR)、X 射线粉末衍射(XRD)和扫描电子显微镜研究了利用波纹巴非蛤乙醇提取物的绿色合成银纳米粒子(AgNPs)及其生物活性。结果表明,通过颜色从绿色变为深褐色来确认合成的纳米粒子。XRD 分析证实 AgNPs 是结晶的,并发现它们的紫外最大特定吸收在 200 到 400nm 之间,场发射扫描电子显微镜的尺寸在 60 到 69nm 之间。FTIR 研究确定了参与 AgNPs 潜在封端的不同功能基团。AgNPs 的抗糖尿病活性通过抑制碳水化合物消化酶(α-葡萄糖苷酶和淀粉酶)来测试。此外,它对乳腺癌细胞(MDF7)表现出潜在的抗癌活性。因此,本研究结果证明了在合成 AgNPs 中使用环保且高效的方法,AgNPs 可以作为生物医学应用的替代生物材料。

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