Nanchang University Modern Agriculture Research Institute, Key Laboratory of Aquatic Resources and Utilization of Jiangxi, School of Life Science, Nanchang University, Nanchang, China.
Nanchang University Modern Agriculture Research Institute, Key Laboratory of Aquatic Resources and Utilization of Jiangxi, School of Life Science, Nanchang University, Nanchang, China.
Ecotoxicol Environ Saf. 2023 Mar 1;252:114589. doi: 10.1016/j.ecoenv.2023.114589. Epub 2023 Jan 30.
Selenium plays a vital role in cancer prevention, antioxidation, and the growth of humans and other vertebrates. Excessive selenium can cause liver injury and metabolic disorders, which can lead to hepatic disease, but few studies have shown the effects of excessive selenium on liver development and its mechanism in zebrafish embryos. In this study, liver development and glucolipid metabolism were investigated in selenium-stressed zebrafish embryos. Under selenium treatment, transgenic fabp10a-eGFP zebrafish embryos showed reduced liver size, and wild-type zebrafish embryos exhibited steatosis and altered lipid metabolism-related indexes and glucose metabolism-related enzyme activities. In addition, selenium-stressed embryos exhibited damaged mitochondria and inhibited autophagy in the liver. An autophagy inducer (rapamycin) alleviated selenium-induced liver injury and restored the expression of some genes related to liver development and glucolipid metabolism. In summary, our research evaluated liver developmental toxicity and metabolic disorders under selenium stress, and confirmed that autophagy and oxidative stress might involve in the selenium-induced hepatic defects.
硒在癌症预防、抗氧化和人类和其他脊椎动物的生长中起着至关重要的作用。过量的硒会导致肝损伤和代谢紊乱，从而导致肝病，但很少有研究表明过量的硒对斑马鱼胚胎肝脏发育的影响及其机制。在这项研究中，研究了硒胁迫斑马鱼胚胎中的肝脏发育和糖脂代谢。在硒处理下，转 fabp10a-eGFP 斑马鱼胚胎的肝脏体积减小，而野生型斑马鱼胚胎则表现出脂肪变性和改变的脂质代谢相关指标以及葡萄糖代谢相关酶活性。此外，硒胁迫的胚胎显示出受损的线粒体和抑制的肝脏自噬。自噬诱导剂（雷帕霉素）缓解了硒引起的肝损伤，并恢复了一些与肝发育和糖脂代谢相关的基因的表达。总之，我们的研究评估了硒胁迫下的肝脏发育毒性和代谢紊乱，并证实自噬和氧化应激可能参与了硒诱导的肝缺陷。
