Luo Yue-Chuan, Chen Chen, Wu Dan-Dan, Lu Jia-Le, Sha Li-Na, Fan Xing, Cheng Yi-Ran, Kang Hou-Yang, Wang Yi, Zhou Yong-Hong, Zhang Chang-Bing, Zhang Hai-Qin
Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China.
Cytogenet Genome Res. 2022;162(6):334-344. doi: 10.1159/000527781. Epub 2023 Feb 1.
Natural hybridization has been frequently observed in Triticeae; however, few studies have investigated the origin of natural intergeneric Triticeae hybrids. In the present study, we discovered three putative hybrid Triticeae plants in the Western Sichuan Plateau of China. Morphologically, the putative hybrids were intermediate between Kengyilia melanthera (2n = 6x = 42; StStYYPP) and Campeiostachys dahurica var. tangutorum (2n = 6x = 42; StStYYHH) with greater plant height and tiller number. Cytological analyses demonstrated that the hybrids were hexaploid with 42 chromosomes (2n = 6x = 42). At metaphase I, 12.10-12.58 bivalents and 13.81-14.18 univalents per cell were observed in the hybrid plants. Genomic in situ hybridization demonstrated that the hybrids had StStYYHP genomes. Phylogenetic analysis of Acc1 sequences indicated that the hybrids were closely related to K. melanthera and C. dahurica var. tangutorum. Our morphological, cytological, and molecular analyses indicate that these hexaploid natural hybrid plants may be hybrids of K. melanthera and C. dahurica var. tangutorum.
在小麦族中经常观察到自然杂交现象；然而，很少有研究调查小麦族属间自然杂交种的起源。在本研究中，我们在中国四川西部高原发现了三株疑似杂交的小麦族植物。从形态上看，这些疑似杂交种介于黑药仲彬草（2n = 6x = 42；StStYYPP）和唐古特新麦草变种（2n = 6x = 42；StStYYHH）之间，株高和分蘖数更多。细胞学分析表明，这些杂交种是具有42条染色体的六倍体（2n = 6x = 42）。在中期I，在杂交植株中观察到每个细胞有12.10 - 12.58个二价体和13.81 - 14.18个单价体。基因组原位杂交表明，这些杂交种具有StStYYHP基因组。对Acc1序列的系统发育分析表明，这些杂交种与黑药仲彬草和唐古特新麦草变种密切相关。我们的形态学、细胞学和分子分析表明，这些六倍体自然杂交植株可能是黑药仲彬草和唐古特新麦草变种的杂交种。
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