Department of Laboratory Haematology, ICPMR, Westmead Hospital, NSW Health Pathology, Westmead, NSW, Australia; Sydney Medical School, Faculty of Medicine and Health, University of Sydney, Camperdown, NSW, Australia.
Flow Cytometry Unit, ICPMR, NSW Health Pathology, Westmead Hospital, Westmead, NSW, Australia.
Pathology. 2023 Apr;55(3):383-390. doi: 10.1016/j.pathol.2022.10.007. Epub 2022 Dec 30.
Measurable residual disease (MRD) monitoring in acute myeloid leukaemia (AML) is becoming increasingly important and is predominantly performed by multiparameter flow cytometry (MFC) or quantitative polymerase chain reactions (RT-qPCR). We investigated the use of multidimensional plots (MD-MFC) for AML MRD monitoring in an adult cohort. AML MRD was determined using a novel MD-MFC method for 115 MRD samples. Results were correlated with traditional two-dimensional MFC (2D-MFC) and molecular methods. Using the standard cut-off of 0.1% CD45+ cells, concordance was 99/115 (p=0.332). Eighty-four of 115 were concordant using a very low reporting limit of 0.01% (p=0.216). MRD <0.1% by either method was present in 40 of 115 samples. Fifteen of 40 were MD-MFC positive and 2D-MFC negative. Of these two of 15 had a molecular MRD marker and both were positive. Molecular MRD markers were available in 36 of 115 cases. Twenty-one of 36 (58%) were concordant with MD-MFC. Eight of 36 had detectable molecular MRD only and eight of 36 had positive MD-MFC only. There was no correlation between either the MFC method and the molecular results. In summary, there is good correlation between MD- and 2D-MFC-MRD and no correlation between the MFC and molecular methods.
在急性髓系白血病（AML）中，可测量残留疾病（MRD）监测变得越来越重要，主要通过多参数流式细胞术（MFC）或实时定量聚合酶链反应（RT-qPCR）进行。我们研究了多维图（MD-MFC）在成人队列中用于 AML MRD 监测的应用。使用一种新的 MD-MFC 方法对 115 个 MRD 样本进行 AML MRD 测定。结果与传统二维 MFC（2D-MFC）和分子方法相关联。使用 0.1% CD45+细胞的标准截止值，99/115（p=0.332）个样本一致。使用非常低的报告限值 0.01%，84/115（p=0.216）个样本一致。两种方法均为 MRD<0.1%的样本为 40/115。这 40 个样本中有 15 个 MD-MFC 阳性而 2D-MFC 阴性。这 15 个中有 2 个分子 MRD 标志物呈阳性，且均为阳性。在 115 个病例中有 36 个有分子 MRD 标志物。36 个中有 21 个与 MD-MFC 一致。36 个中有 8 个仅有可检测到的分子 MRD，36 个中有 8 个仅有阳性 MD-MFC。MFC 方法与分子结果之间没有相关性。总之，MD-MFC 和 2D-MFC-MRD 之间相关性良好，而 MFC 和分子方法之间没有相关性。
