Chakrabarti S, Tuchweber B
Département de Médecine du Travail et Hygiène du Milieu, Université de Montréal, Que. Canada.
Toxicol Lett. 1987 Nov;39(1):27-34. doi: 10.1016/0378-4274(87)90253-0.
Adult male Sprague-Dawley rats were treated simultaneously with 4 mmol styrene per kg i.p., twice a day at an interval of 4 h, and 10 mmol toluene per kg once a day, 5 days a week for 4 consecutive weeks. After the last day of treatment, the rats were placed in metabolism cages for collection of urines for 24 h and then were sacrificed. Such mixed exposure produced significant increases in the urinary excretion of gamma-glutamyl transpeptidase, glucose and proteins as compared to those with either solvent alone. An increase, but not significant, in the urinary excretion of N-acetyl-beta-D-glucosaminidase was also noticed due to such exposure. Electron microscopic examination of renal cortex 24 h after the mixed exposure showed the appearance of many vacuoles of various sizes surrounded by a single membrane, which were not seen in rats treated with either styrene or toluene alone. Metabolism studies showed only a significant increase in the urinary excretion of hippuric acid due to mixed exposure. These data indicate that under certain conditions, mixed subchronic exposure to styrene and toluene may have the potential to increase further the nephrotoxic response as compared to that of either solvent alone.
成年雄性Sprague-Dawley大鼠，腹腔注射给予每千克体重4毫摩尔苯乙烯，每天两次，间隔4小时，同时每周5天、连续4周每天一次给予每千克体重10毫摩尔甲苯。在末次给药后的当天，将大鼠置于代谢笼中收集24小时尿液，然后处死。与单独使用任一溶剂相比，这种混合暴露使γ-谷氨酰转肽酶、葡萄糖和蛋白质的尿排泄量显著增加。由于这种暴露，还观察到N-乙酰-β-D-氨基葡萄糖苷酶的尿排泄量增加，但不显著。混合暴露24小时后对肾皮质进行电子显微镜检查，可见许多大小各异的空泡，周围有单层膜，单独给予苯乙烯或甲苯的大鼠未见此种情况。代谢研究表明，混合暴露仅使马尿酸的尿排泄量显著增加。这些数据表明，在某些条件下，与单独使用任一溶剂相比，苯乙烯和甲苯的混合亚慢性暴露可能有进一步增加肾毒性反应的可能性。
