Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Department of Microbiology, All India Institute of Medical Sciences (AIIMS) Bilaspur, Himachal Pradesh, India.
Ocul Immunol Inflamm. 2024 Aug;32(6):976-982. doi: 10.1080/09273948.2023.2170888. Epub 2023 Feb 1.
Truenat MTB Plus assay was evaluated for diagnosing ocular tuberculosis (OTB) and detecting multi-drug resistant (MDR) and extremely-drug resistant (XDR) OTB.
A total of 75 vitreous fluid specimens [five confirmed OTB, 40 clinically suspected OTB and 30 controls] were subjected to Truenat MTB Plus, multiplex PCR, and Xpert Ultra. Chips of Truenat were used for detecting rifampicin, isoniazid, fluoroquinolone and bedaquiline resistance. The performance was compared against culture, composite reference standard, and gene sequencing.
The overall sensitivity of TruePlus, MPCR, and Ultra in diagnosing OTB was 66.6%, 73.3%, and 55.5%, respectively. Out of six cases with mutations in rpoB gene, RifR was detected in five by TrueRif and four by Ultra. Three MDR and one XDR-OTB were reported by Truenat.
Truenat assay along with its strategic chips is a rapid and reliable tool for diagnosis of OTB and detection of drug resistance, including MDR and XDR-OTB. OTB: Ocular tuberculosis; XDR: Extremely drug resistant; Ultra: Xpert MTB/RIF Ultra; Xpert: Xpert MTB/RIF; PCR: polymerase chain reaction; NAATs: Nucleic acid amplification tests; MDR: Multi Drug Resistant; NSP: National Strategic plan for elimination of tuberculosis; FqR: Fluoroquinolone resistant; BdqR: bedaquiline resistant; TrueRif: Truenat MTB Rif Dx; TruePlus: Truenat Plus; INH: Isoniazid; DST: Drug susceptibility testing; MGIT: Mycobacterial growth indicator tube; CRF: Composite reference standard; PPV: positive predictive value; NPV: negative predictive value; EPTB: extrapulmonary tuberculosis; VF: vitreous fluid; DNA: deoxyribonucleic acid; ATT: antitubercular therapy; RifR: Rifampicin resistance; RifS: Rifampicin susceptible; RifI: Rifampicin indeterminate.
Truenat MTB Plus 检测法用于诊断眼部结核（OTB），并检测耐多药（MDR）和极端耐药（XDR）OTB。
共 75 份玻璃体液标本[5 份确诊 OTB、40 份临床疑似 OTB 和 30 份对照]进行了 Truenat MTB Plus、多重 PCR 和 Xpert Ultra 检测。Truenat 的芯片用于检测利福平、异烟肼、氟喹诺酮和贝达喹啉耐药性。性能与培养物、综合参考标准和基因测序进行了比较。
TruePlus、MPCR 和 Ultra 诊断 OTB 的总体敏感性分别为 66.6%、73.3%和 55.5%。在 rpoB 基因发生突变的 6 例中，有 5 例通过 TrueRif 检测到 RifR，4 例通过 Ultra 检测到 RifR。Truenat 报告了 3 例 MDR 和 1 例 XDR-OTB。
Truenat 检测法及其战略芯片是一种快速可靠的工具，可用于诊断 OTB 和检测耐药性，包括 MDR 和 XDR-OTB。OTB：眼部结核；XDR：极端耐药；Ultra：Xpert MTB/RIF Ultra；Xpert：Xpert MTB/RIF；PCR：聚合酶链反应；NAATs：核酸扩增试验；MDR：多药耐药；NSP：国家结核病消除战略计划；FqR：氟喹诺酮耐药；BdqR：贝达喹啉耐药；TrueRif：Truenat MTB Rif Dx；TruePlus：Truenat Plus；INH：异烟肼；DST：药物敏感性试验；MGIT：分枝杆菌生长指示管；CRF：综合参考标准；PPV：阳性预测值；NPV：阴性预测值；EPTB：肺外结核病；VF：玻璃体；DNA：脱氧核糖核酸；ATT：抗结核治疗；RifR：利福平耐药；RifS：利福平敏感；RifI：利福平不确定。
