Department of Industrial and Environmental Biotechnology, National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
J Biomed Mater Res B Appl Biomater. 2023 May;111(5):1121-1132. doi: 10.1002/jbm.b.35219. Epub 2023 Feb 2.
In recent years, cancerous cases have increased remarkably worldwide, and metastasis is the leading cause of death. Therefore, research on the early detection of cancer and metastasis has expanded to aid successful cancer treatment. Here in this paper, at the first step, an electrospun nanofibrous membrane (NFM) with a core-shell structure was fabricated from PCL and HA to achieve cancer cell capturing (about 75% of cells). On the other hand, hyaluronic acid (HA)-functionalized graphene quantum dots (GQDs) were used to detect captured cancer cells on NFM through the changes in photoluminescence intensity. Therefore, CD44 receptor-HA interaction is the main principle used for both entrapment and detection of cancer cells. Results demonstrated the GQD-HA fluorescent intensity of solution decreased through the increase of the captured cancer cell numbers on NFM, which is related to the more adsorption of GQD nanocomposites to the CD44 receptors. In contrast, this intensity for noncancerous cells was steady with any cell concentrations. This difference shows the system's remarkable selectivity and specificity, which can be crucial in fluorescent imaging for accurate cancer diagnosis.
近年来，全球癌症病例显著增加，转移是死亡的主要原因。因此，癌症和转移的早期检测研究已经扩展到帮助成功治疗癌症。在本文中，首先，我们制备了具有核壳结构的 PCL/HA 电纺纳米纤维膜（NFM），以实现癌细胞捕获（约 75%的细胞）。另一方面，我们使用透明质酸（HA）功能化的石墨烯量子点（GQD）通过光致发光强度的变化来检测 NFM 上捕获的癌细胞。因此，CD44 受体-HA 相互作用是用于捕获和检测癌细胞的主要原理。结果表明，随着 NFM 上捕获的癌细胞数量的增加，溶液中的 GQD-HA 荧光强度降低，这与 GQD 纳米复合材料对 CD44 受体的更多吸附有关。相比之下，对于非癌细胞，无论细胞浓度如何，该强度都保持稳定。这种差异表明该系统具有显著的选择性和特异性，这对于荧光成像的准确癌症诊断可能至关重要。
