牛外周血白细胞中糖苷酶的检测与活性
Assays and activities of glycosidic enzymes in bovine peripheral blood leukocytes.
作者信息
Hurley W L
机构信息
Animal Sciences Dept., Univ. of Illinois, Urbana 61801.
出版信息
Vet Immunol Immunopathol. 1987 Sep;16(1-2):85-93. doi: 10.1016/0165-2427(87)90176-0.
Methods are described for the quantitative measurement of N-acetyl-beta-D-glucosaminidase, alpha-mannosidase and beta-glucuronidase in peripheral blood leukocytes of the bovine. Enzyme kinetics and stability were determined. Activities of the glycosidases in polymorphonuclear leukocytes and mononuclear leukocytes were determined using the optimized assays. Polymorphonuclear leukocytes had greater activities of N-acetyl-beta-D-glucosaminidase and alpha-mannosidase, and similar levels of beta-glucuronidase, when compared to mononuclear leukocytes.
描述了定量测定牛外周血白细胞中N - 乙酰 - β - D - 氨基葡萄糖苷酶、α - 甘露糖苷酶和β - 葡萄糖醛酸酶的方法。测定了酶动力学和稳定性。使用优化的测定方法测定多形核白细胞和单核白细胞中糖苷酶的活性。与单核白细胞相比，多形核白细胞具有更高的N - 乙酰 - β - D - 氨基葡萄糖苷酶和α - 甘露糖苷酶活性，以及相似水平的β - 葡萄糖醛酸酶活性。
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