Marshall Andrew P, Carlson Erin E
Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, Minnesota, 55455, USA.
Department of Medicinal Chemistry, University of Minnesota, 208 Harvard Street SE, Minneapolis, Minnesota, 55454, USA.
Chembiochem. 2023 Apr 3;24(7):e202200757. doi: 10.1002/cbic.202200757. Epub 2023 Mar 10.
Streptomyces coelicolor is a prolific producer of natural products and serves as a model organism for their study. It produces several pigmented antibiotics, the best-studied of which are the actinorhodins. We used a combination of liquid chromatography-mass spectrometry (LC-MS) and computational tools used for annotating the detected species (e. g., spectral matching, in-silico predictors, molecular networking) to identify putative new actinorhodin analogs. These studies led to the discovery of the first trimeric benzoisochromanequinone, θ-actinorhodin (1). Further metabolomics analysis revealed that the relative amounts of shunt products produced were similar between the two growth conditions explored. This suggests that, while substantially different products were being produced, the biosynthetic gene clusters were similarly active. Overall, this work describes the discovery of the first trimeric benzoisochromanequinone and explores the biosynthetic processes that might lead to its production by metabolomics analysis of relevant intermediates.
天蓝色链霉菌是天然产物的丰富生产者，并作为研究它们的模式生物。它产生几种有色抗生素，其中研究得最透彻的是放线紫红素。我们结合使用液相色谱 - 质谱联用（LC - MS）和用于注释检测到的物种的计算工具（例如，光谱匹配、计算机预测器、分子网络）来鉴定推定的新型放线紫红素类似物。这些研究导致发现了第一种三聚体苯并异香豆素醌，θ - 放线紫红素（1）。进一步的代谢组学分析表明，在所探索的两种生长条件下，分流产物的相对量相似。这表明，虽然产生的产物有很大不同，但生物合成基因簇的活性相似。总体而言，这项工作描述了第一种三聚体苯并异香豆素醌的发现，并通过对相关中间体的代谢组学分析探索了可能导致其产生的生物合成过程。
