Lions VisionGift, Portland, OR.
Research and Development Department of AL.CHI.MI.A. S.R.L., Ponte San Nicolò (PD), Italy; and.
Cornea. 2023 May 1;42(5):630-638. doi: 10.1097/ICO.0000000000003201. Epub 2022 Dec 12.
The aim of this study was to compare the performance of Kerasave and Optisol-GS for hypothermic corneal storage for 14 days.
This study was a prospective laboratory investigation. Mate corneas were recovered into Kerasave or Optisol-GS (27 pairs) and stored at 2°C to 8°C for 14 days. Corneas were evaluated by trained eye bank technicians, and study parameters were compared between the initial and final evaluations. Endothelial cell density (ECD), hexagonality (HEX), and coefficient of variation (CV) were evaluated by specular microscopy, and central corneal thickness (CCT) was examined by optical coherence tomography after 1, 3, 7, and 14 days of storage. Corneal transparency was scored using slit lamp examination at days 1 and 14.
Average ECD, HEX, and CV for the Kerasave (2653 ± 303 cells/mm 2 , 57 ± 4%, and 36 ± 3%) and Optisol-GS (2623 ± 306 cells/mm 2 , 57 ± 5%, and 36 ± 4%) groups were not significantly different at day 1. There was also no difference at any other study time points (all P > 0.05). ECD did not significantly change from day 1 to day 14 in either group ( P > 0.05), but a statistically significant change in HEX and CV was observed between day 1 and day 14 in both groups ( P < 0.01). Average CCT measured at day 1 for corneas stored in Kerasave was 622 ± 49 μm and those stored in Optisol-GS was 580 ± 35 μm ( P < 0.01). The difference in CCT measurements was not significantly different at day 14 (Kerasave: 674 ± 46 μm vs. Optisol-GS: 647 ± 58 μm, P > 0.05). Corneal transparency was not significantly different between the 2 groups at day 1 or day 14.
The corneal quality and clinically relevant parameters including ECD, endothelial morphometry, and corneal transparency were not different in corneas stored in Kerasave or Optisol-GS for 14 days. The initial difference in CCT between the 2 groups decreased at day 14. These results demonstrated that Kerasave corneal storage solution preserves the corneal endothelium similarly to Optisol-GS.
本研究旨在比较 Kerasave 和 Optisol-GS 在 14 天低温角膜保存中的性能。
这是一项前瞻性实验室研究。将供体角膜分别回收至 Kerasave 或 Optisol-GS（27 对）中，并在 2°C 至 8°C 下储存 14 天。由经过培训的眼库技术人员对角膜进行评估，并比较初始和最终评估之间的研究参数。通过共焦显微镜评估内皮细胞密度（ECD）、六边形（HEX）和变异系数（CV），并在储存后 1、3、7 和 14 天通过光学相干断层扫描检查中央角膜厚度（CCT）。在第 1 天和第 14 天使用裂隙灯检查对角膜透明度进行评分。
Kerasave（2653±303 个细胞/mm 2 、57±4%和 36±3%）和 Optisol-GS（2623±306 个细胞/mm 2 、57±5%和 36±4%）组的平均 ECD、HEX 和 CV 在第 1 天均无显著差异。在其他任何研究时间点也没有差异（均 P>0.05）。在这两个组中，从第 1 天到第 14 天，ECD 均无显著变化（P>0.05），但在这两个组中，HEX 和 CV 在第 1 天和第 14 天之间均观察到统计学显著变化（P<0.01）。Kerasave 储存的角膜在第 1 天的平均 CCT 为 622±49μm，Optisol-GS 储存的角膜为 580±35μm（P<0.01）。第 14 天 CCT 测量值的差异无统计学意义（Kerasave：674±46μm 对比 Optisol-GS：647±58μm，P>0.05）。在第 1 天或第 14 天，两组之间的角膜透明度无显著差异。
在 14 天的 Kerasave 或 Optisol-GS 储存中，角膜质量和临床相关参数（包括 ECD、内皮形态计量学和角膜透明度）没有差异。两组之间在第 14 天 CCT 的初始差异减小。这些结果表明，Kerasave 角膜储存液可与 Optisol-GS 一样有效地保存角膜内皮。
