State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, China.
State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, China; College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, China.
Environ Pollut. 2023 Apr 1;322:121143. doi: 10.1016/j.envpol.2023.121143. Epub 2023 Jan 30.
Tetrabromobisphenol A (TBBPA) is an industrial chemical and the most widely used brominated flame retardant, and has raised environmental health concerns. However, the maternal transfer toxicity of TBBPA is less studied in fish despite its frequency in the water environment, and limited evidence exists to confirm the major contributing factors. In this study, we performed a 28-d experiment on female and male zebrafish exposed to TBBPA (0, 5, 50, and 500 μg/L), and shortened body length of offspring larvae was observed at the maximum exposure concentration. By cross-mating control and exposed zebrafish (male or female), our results showed that the observed growth inhibition in the progeny was attributed to the maternal transfer effect. Although 28-d exposure resulted in the existence of TBBPA in ovaries and ova, the maternal transfer of TBBPA was not responsible for the shortened body length of offspring larvae, as evidenced through TBBPA embryo microinjection. Moreover, proteomic analyses in ova indicated that the abundance of apolipoproteins (apoa1, apoa1b, apoa2, apoa4b, and apoc1) was significantly downregulated in the ova, which may be partially responsible for the shortened body length of offspring larvae. Interestingly, these proteins did not differentially express in the ovaries. Therefore, our results demonstrate that TBBPA exposure disturbed maternal protein transfer from the ovaries to the ova, providing novel insights into the underlying maternal transfer effects.
四溴双酚 A（TBBPA）是一种工业化学品，也是应用最广泛的溴系阻燃剂，对环境健康造成了一定的威胁。尽管 TBBPA 在水环境中很常见，但鱼类的母体转移毒性研究较少，且存在的证据有限，无法确定主要的影响因素。在这项研究中，我们对暴露于 TBBPA（0、5、50 和 500μg/L）的雌性和雄性斑马鱼进行了 28 天的实验，在最大暴露浓度下观察到子代幼鱼的体长缩短。通过杂交对照和暴露于 TBBPA 的斑马鱼（雄性或雌性），我们的结果表明，观察到的子代生长抑制归因于母体转移效应。尽管 28 天的暴露导致卵巢和卵子中存在 TBBPA，但 TBBPA 的母体转移并不是导致子代幼鱼体长缩短的原因，这可以通过 TBBPA 胚胎微注射得到证明。此外，卵子中的蛋白质组学分析表明，载脂蛋白（apoa1、apoa1b、apoa2、apoa4b 和 apoc1）的丰度在卵子中显著下调，这可能是子代幼鱼体长缩短的部分原因。有趣的是，这些蛋白质在卵巢中没有差异表达。因此，我们的结果表明，TBBPA 暴露干扰了母体蛋白从卵巢向卵子的转移，为母体转移效应的潜在机制提供了新的见解。
Zotero 插件