Kageyama A, Fukata T, Baba E, Arakawa A
Department of Veterinary Medicine, College of Agriculture, University of Osaka Prefecture, Japan.
Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Jul;265(3-4):353-9. doi: 10.1016/s0176-6724(87)80253-5.
Monoflora chickens were established at the age of 2 days by an oral inoculation of one of six species of bacteria (Lactobacillus acidophilus, Bifidobacterium thermophilum, Bacteroides vulgatus, Clostridium perfringens, Escherichia coli, or Streptococcus faecalis). Two days later the chickens were infected with Eimeria tenella (5 X 10(4) oocytes per bird). There were four groups: uninfected birds as controls, the birds infected with either bacteria or E. tenella alone, and the birds infected with bacteria and E. tenella. As observed under the scanning electron microscope (SEM) seven days after E. tenella infection, damage of the cecal mucosa in all groups infected with bacteria + E. tenella was more severe than in that infected with E. tenella alone. Most severe damage to the cecal mucosa was found in the birds infected with either C. perfringens or B. thermophilum combined with E. tenella.
在2日龄时通过口服接种六种细菌之一（嗜酸乳杆菌、嗜热双歧杆菌、普通拟杆菌、产气荚膜梭菌、大肠杆菌或粪肠球菌）建立单菌群鸡。两天后，给鸡感染柔嫩艾美耳球虫（每只鸡5×10⁴个卵囊）。有四组：未感染的鸡作为对照，单独感染细菌或柔嫩艾美耳球虫的鸡，以及同时感染细菌和柔嫩艾美耳球虫的鸡。在柔嫩艾美耳球虫感染七天后通过扫描电子显微镜（SEM）观察发现，所有感染细菌+柔嫩艾美耳球虫的组中盲肠黏膜的损伤比单独感染柔嫩艾美耳球虫的组更严重。在感染产气荚膜梭菌或嗜热双歧杆菌并同时感染柔嫩艾美耳球虫的鸡中发现盲肠黏膜损伤最严重。
