Zhu Xuyou, Zhu Peipei, Chen Xue, Zhang Long, Wu Caixia, Zhang Haoyang, Shen Xiaoying, Qi Yao, Chen Mingmin, Wang Shunli, Yi Xianghua
Department of Pathology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Shanghai OUTDO Biotech Co. Ltd. Shanghai, China.
Histol Histopathol. 2023 Nov;38(11):1327-1335. doi: 10.14670/HH-18-590. Epub 2023 Jan 23.
In recent years, 3'-phosphoadenosine-5'-phosphosulfate synthase 1 (PAPSS1) has been found to be highly expressed in some cancers and significantly associated with prognosis. Nevertheless, the role of PAPSS1 in esophageal squamous cell carcinoma (ESCC) is poorly understood.
In this study, PAPSS1 expression in ESCC samples was researched through real-time quantitative polymerase chain reaction (qPCR), immunohistochemistry (IHC), and western blot (WB) techniques. siRNA technology was then used to inhibit PAPSS1 expression in ESCC cells, and cytologic tests were conducted to research gene affection on cell apoptosis, proliferation, and migration. Then, the expression of Bcl2, Ki67, and Snail was detected using qPCR and WB tests. These experimental data were analyzed by GraphPad software, where the P-value<0.05 was statistically significant.
The results showed that PAPSS1 expression level in ESCC tissues was higher than in the adjacent tissues. The data also showed that PAPSS1 was significantly correlated with N stage, and that the patients with high expressions had longer survival time. After transfection for 48 hours, the cell apoptosis rate of siRNA-PAPSS1 transfected groups decreased significantly, whereas the cell proliferation rate and migration ability increased relative to the control. At the same time, the expression levels of Bcl2, Ki67 and Snail were all upregulated by siRNA-PAPSS1. PAPSS1, however, was suppressed.
PAPSS1 may be an ESCC suppressor gene, and its specific molecular mechanism in ESCC needs to be further studied.
近年来，已发现3'-磷酸腺苷-5'-磷酸硫酸合成酶1（PAPSS1）在某些癌症中高表达，并与预后显著相关。然而，PAPSS1在食管鳞状细胞癌（ESCC）中的作用尚不清楚。
在本研究中，通过实时定量聚合酶链反应（qPCR）、免疫组织化学（IHC）和蛋白质印迹（WB）技术研究了ESCC样本中PAPSS1的表达。然后使用siRNA技术抑制ESCC细胞中PAPSS1的表达，并进行细胞学试验以研究该基因对细胞凋亡、增殖和迁移的影响。随后，使用qPCR和WB试验检测Bcl2、Ki67和Snail的表达。这些实验数据通过GraphPad软件进行分析，其中P值<0.05具有统计学意义。
结果显示，ESCC组织中PAPSS1的表达水平高于相邻组织。数据还表明，PAPSS1与N分期显著相关，高表达患者的生存时间更长。转染48小时后，siRNA-PAPSS1转染组的细胞凋亡率显著降低，而细胞增殖率和迁移能力相对于对照组增加。同时，siRNA-PAPSS1使Bcl2、Ki67和Snail的表达水平均上调。然而，PAPSS1受到抑制。
PAPSS1可能是一种ESCC抑制基因，其在ESCC中的具体分子机制有待进一步研究。
