偏头痛患者中散布重复序列的 DNA 甲基化状态。
DNA methylation status of interspersed repetitive sequences in patients with migraine.
机构信息
Department of Molecular and Medical Genetics, Tbilisi State Medical University, Tbilisi, Georgia.
Department of Neurology, Tbilisi Institute of Medicine, Tbilisi, Georgia.
出版信息
J Int Med Res. 2023 Feb;51(2):3000605231152109. doi: 10.1177/03000605231152109.
OBJECTIVE
To analyse the methylation status of the Long Interspersed Nuclear Element-1 (LINE-1) and Short Interspersed Nuclear Element Alu (Alu) of peripheral blood mononuclear cells (PBMCs) from patients with migraine compared with healthy control subjects.
METHODS
This case-control study recruited patients with migraine without aura and age-matched healthy control subjects. PBMCs were purified from peripheral blood samples. Methylation levels and patterns of LINE-1 and Alu sequences were evaluated using combined bisulfite restriction analysis-interspersed repetitive sequences polymerase chain reaction.
RESULTS
A total of 84 patients with migraine and 82 age-matched healthy controls were enrolled in the study. High levels of unmethylated cytosines in both the LINE-1 and Alu repetitive elements were observed in the migraine group compared with the control subjects. In addition, a significant difference was detected in the methylation level of LINE-1 between TT and CC genotype groups of the methylenetetrahydrofolate reductase gene.
CONCLUSIONS
These results suggest that analysis of epigenetic biomarkers in PBMCs may help to identify patients at a higher risk of migraine development.
目的
分析偏头痛患者外周血单个核细胞（PBMC）中长散布核元件-1（LINE-1）和短散布核元件 Alu（Alu）的甲基化状态，并与健康对照组进行比较。
方法
本病例对照研究招募了无先兆偏头痛患者和年龄匹配的健康对照者。从外周血样本中纯化 PBMC。采用联合亚硫酸氢盐限制性分析-散布重复序列聚合酶链反应评估 LINE-1 和 Alu 序列的甲基化水平和模式。
结果
共纳入 84 例偏头痛患者和 82 名年龄匹配的健康对照者。与对照组相比，偏头痛组中 LINE-1 和 Alu 重复元件的未甲基化胞嘧啶水平较高。此外，亚甲基四氢叶酸还原酶基因 TT 和 CC 基因型组的 LINE-1 甲基化水平存在显著差异。
结论
这些结果表明，分析 PBMC 中的表观遗传生物标志物可能有助于识别偏头痛发病风险较高的患者。
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