凝溶胶蛋白通过增强肿瘤坏死因子受体 2/半胱氨酸天冬氨酸蛋白酶 10（作为死亡受体通路）的表达抑制结肠癌细胞的增殖。

Gelsolin Inhibits the Proliferation of Colon Cancer Cells by Enhancing the Expression of TNFR2/CASP10 as a Death Receptor Pathway.

机构信息

Department of Microbiology, The Heilongjiang Key Laboratory of Immunity and Infection, Harbin Medical University, Harbin, 150081, China.

出版信息

Protein Pept Lett. 2023;30(3):214-220. doi: 10.2174/0929866530666230124113639.

DOI:10.2174/0929866530666230124113639

PMID:36734906

Abstract

BACKGROUND

Colon cancer has the second highest incidence rate of digestive system tumors. It relies on surgical treatment, radiotherapy and chemotherapy, and targeted drug therapy.

OBJECTIVE

To study the mechanism of GSN in the proliferation of colon cancer cells.

MATERIALS AND METHODS

The expression of gelsolin (GSN) was analyzed with the data of colon cancer patients in the TCGA database. SW620 cells were treated by GSN in vitro and the gene expression was detected by immunoblotting and quantitative PCR.

RESULTS

The expression of GSN was found significantly low in colon cancer cells and correlated with the prognosis of patients. The SW620 cell line cultured in vitro was treated with exogenous GSN. SW620 can be significantly inhibited above the concentration of 250 μg/ml. The results of immunoblotting and quantitative PCR showed that exogenous GSN can effectively improve the transcription level of death receptor-related pathway genes such as TNFR2 and CASP10.

CONCLUSION

This study found that GSN inhibited the proliferation of SW620 cells in vitro by upregulating the expression of death receptor pathway-related proteins.

摘要

背景

结肠癌是消化系统肿瘤中发病率第二高的肿瘤。它依赖于手术治疗、放化疗和靶向药物治疗。

目的

研究凝溶胶蛋白（GSN）在结肠癌细胞增殖中的作用机制。

材料和方法

利用 TCGA 数据库中结肠癌患者的数据来分析凝溶胶蛋白（GSN）的表达。通过体外实验用 GSN 处理 SW620 细胞，并用免疫印迹和定量 PCR 检测基因表达。

结果

发现 GSN 在结肠癌细胞中的表达明显降低，与患者的预后相关。在体外培养的 SW620 细胞系中，用外源性 GSN 处理。SW620 在浓度高于 250μg/ml 时可被明显抑制。免疫印迹和定量 PCR 的结果表明，外源性 GSN 可以有效提高 TNFR2 和 CASP10 等死亡受体相关通路基因的转录水平。