Deng Xiujuan, Wu Haigen, Xiong Liping, Wu Mouxi, Cao Jinfu, Liu Jing, Xie Wenyang
Department of Gynaecology, Jiujiang Maternal and Child Health Hospital. Jiujiang, Jiangxi 332000, China.
Department of Obstetrics and Gynecology, De'an County People's Hospital. Jiujiang, Jiangxi 330499, China.
Acta Biochim Pol. 2023 Feb 3;70(1):169-174. doi: 10.18388/abp.2020_6412.
To investigate the function and possible mechanism of miR-92a in malignant behaviors such as paclitaxel resistance in ovarian cancer (OC) cells. The miR-92a and PTEN expression were detected by real-time PCR (RT-PCR). The cell viability and apoptosis were detected by MTT, colony formation and flow cytometry assay, respectively. Dual-luciferase reporter assay was adopted to verify the targeting relationship between miR-92a and PTEN. Besides, we measured the relative protein levels of PTEN and p-AKT/AKT by Western blot. MiR-92a was significantly highly expressed in OC cells, and its high expression could notably enhance paclitaxel resistance, cell proliferation and colony formation, as well as inhibit apoptosis in SKOV3-Tax cells. Further luciferase reporter assay and expression detection showed that miR-92a could target and regulate PTEN and that there was a targeted relationship between them. In addition, further exploration of the mechanism revealed that miR-92a regulated PTEN/Akt signaling pathway. MiR-92a not only promotes the proliferation, colony formation and paclitaxel resistance of SKOV3-Tax cells in OC, but also inhibits apoptosis, and it may be related to the regulation of the PTEN/Akt signaling pathway. MiR-92a serves as a potential biomarker for the malignant biological behavior of OC cells.
研究miR-92a在卵巢癌（OC）细胞紫杉醇耐药等恶性行为中的作用及可能机制。采用实时荧光定量PCR（RT-PCR）检测miR-92a和PTEN的表达。分别通过MTT法、集落形成实验和流式细胞术检测细胞活力和凋亡情况。采用双荧光素酶报告基因实验验证miR-92a与PTEN之间的靶向关系。此外，通过蛋白质免疫印迹法检测PTEN和p-AKT/AKT的相对蛋白水平。miR-92a在OC细胞中显著高表达，其高表达可显著增强SKOV3-Tax细胞的紫杉醇耐药性、细胞增殖和集落形成能力，并抑制细胞凋亡。进一步的荧光素酶报告基因实验和表达检测表明，miR-92a可靶向调控PTEN，二者存在靶向关系。此外，机制研究发现miR-92a可调控PTEN/Akt信号通路。miR-92a不仅促进OC中SKOV3-Tax细胞的增殖、集落形成和紫杉醇耐药性，还抑制细胞凋亡，这可能与调控PTEN/Akt信号通路有关。miR-92a可作为OC细胞恶性生物学行为的潜在生物标志物。
