两种真空瓶血液培养基的评估——补充蛋白胨肉汤和脑心浸液肉汤。

Evaluation of two vacuum bottle blood culture media--supplemented peptone broth and brain heart infusion broth.

作者信息

Sandven P, Høiby E A, Vorland L

机构信息

National Institute of Public Health, Oslo, Norway.

出版信息

Acta Pathol Microbiol Immunol Scand B. 1987 Aug;95(4):257-9. doi: 10.1111/j.1699-0463.1987.tb03122.x.

DOI:10.1111/j.1699-0463.1987.tb03122.x

PMID:3673582

Abstract

A new blood culture medium (brain heart infusion broth) (BHI) from Terumo was compared with a commonly used blood culture medium, supplemented peptone broth (Becton Dickinson) (SPB). Blood from patients with suspected bacteraemia was simultaneously cultured in the two media, approximately five ml of blood in each bottle. Both bottles were incubated aerobically at 37 degrees C for 7 days. A total of 3724 paired blood cultures (sets) were processed. Altogether 340 (9.1%) of the blood culture sets were positive. Of these, 233 were positive in both media, 51 in the SPB medium only and 56 in the BHI medium only. Despite the similar overall results, Streptococcus spp. and anaerobic bacteria were detected more frequently in the SPB bottles and Enterobacteriaceae more frequently in the BHI bottles.

摘要

将来自泰尔茂公司的一种新型血培养基（脑心浸液肉汤）（BHI）与一种常用血培养基——补充蛋白胨肉汤（BD公司）（SPB）进行比较。对疑似菌血症患者的血液同时在这两种培养基中培养，每个瓶中加入约5毫升血液。两个瓶子均在37℃有氧条件下培养7天。共处理了3724对血培养（组）。总共有340（9.1%）组血培养呈阳性。其中，233组在两种培养基中均呈阳性，51组仅在SPB培养基中呈阳性，56组仅在BHI培养基中呈阳性。尽管总体结果相似，但在SPB瓶中更频繁地检测到链球菌属和厌氧菌，而在BHI瓶中更频繁地检测到肠杆菌科细菌。