Sandven P, Høiby E A
Acta Pathol Microbiol Immunol Scand B. 1985 Jun;93(3):233-6. doi: 10.1111/j.1699-0463.1985.tb02881.x.
Blood from patients with suspected bacteraemia was simultaneously cultured in two Vacutainer blood culture bottles containing 45 ml of supplemented peptone broth (Becton Dickinson), five ml of blood in each bottle. The cultures were incubated at 37 degrees C for seven days. One of the bottles was vented and the other unvented. A total of 3958 blood culture sets were processed during the 20-month period of the study. Altogether 438 (11%) of the blood culture sets were positive. Of the 438 isolates, 317 (72%) were detected in both bottles, 61 (14%) in the vented bottle and 60 (14%) in the unvented bottle. A total of 60 (14%) isolates would not have been detected if only five ml of blood had been cultured. Only minor differences in the isolation rates of different bacterial and fungal species from the vented and unvented bottles were found. The results indicate that aerobic incubation of Vacutainer bottles with supplemented peptone broth is sufficient in routine diagnostic work.
将疑似菌血症患者的血液同时接种于两个含有45毫升补充蛋白胨肉汤（Becton Dickinson公司生产）的Vacutainer血培养瓶中，每个瓶中接种5毫升血液。培养物在37℃下孵育7天。其中一个瓶通气，另一个瓶不通气。在研究的20个月期间，共处理了3958套血培养标本。总共有438套（11%）血培养标本呈阳性。在438株分离菌中，317株（72%）在两个瓶中均被检测到，61株（14%）在通气瓶中被检测到，60株（14%）在不通气瓶中被检测到。如果仅培养5毫升血液，总共60株（14%）分离菌将不会被检测到。在通气瓶和不通气瓶中，不同细菌和真菌种类的分离率仅存在微小差异。结果表明，在常规诊断工作中，使用补充蛋白胨肉汤的Vacutainer瓶进行需氧培养就足够了。
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