Saad Abeer Ezzat, Othman Ahmad Aly, Ghanem Heba Bassiony, Soliman Shaimaa, Alshenawy Hanan Alsaeid, Ghafar Muhammad Tarek Abdel, Rayia Dina Moustafa Abou
Medical Parasitology Department, Faculty of Medicine, Tanta University, Egypt.; Medical Parasitology Sub-Unit, Pathology Department, College of Medicine, Jouf University, Sakaka, Saudi Arabia.
Medical Parasitology Department, Faculty of Medicine, Tanta University, Egypt.
Parasitol Int. 2023 Jun;94:102737. doi: 10.1016/j.parint.2023.102737. Epub 2023 Feb 1.
Nutritional supplements, particularly vitamin D, have been widely used worldwide in the treatment of various infections, including parasites. This study aimed to evaluate the potential effects of vitamin D3 supplementation on the muscular phase of trichinellosis in experimental animals. Mice were divided as follows: (group I): infected untreated, (group IIa) infected and treated with vitamin D3 for 12 doses beginning 2 weeks before infection and continuing after infection, (group IIb) infected and treated with vitamin D3 for 8 doses beginning on the same day of infection, (group III) normal control, (group IVa) which received vitamin D3 for 12 doses and (group IVb) which received vitamin D3 for 8 doses. Mice were sacrificed 35 days after infection and total muscle larval count, and histopathological examination of muscle samples with immunohistochemical staining of cyclooxygenase 2 (COX2) and inducible nitric oxide synthase (iNOS) were performed. Muscle relative cathelicidin mRNA expression was assessed, as well as serum levels of muscle enzymes CK and LDH, interleukin-4 (IL-4), IL-10, IL-17 and interferon-gamma (INF-γ). Vitamin D3 supplementation significantly reduced muscle larval count, inflammatory cellular infiltration, COX2 and iNOS expression. Furthermore, it increased cathelicidin gene expression, decreased serum levels of CK and LDH and affected serum cytokine levels, increasing serum IL-4 and IL10 levels while decreasing serum INF γ and IL-17. In conclusion, vitamin D3 supplementation has favorable outcomes on the muscle phase of trichinellosis, including anti-inflammatory, antioxidant, and immunomodulatory effects.
营养补充剂，尤其是维生素D，在全球范围内已被广泛用于治疗包括寄生虫感染在内的各种感染。本研究旨在评估补充维生素D3对实验动物旋毛虫病肌肉期的潜在影响。小鼠分为以下几组：（第一组）：感染未治疗组；（第二组a）在感染前2周开始用维生素D3治疗12剂，并在感染后继续治疗；（第二组b）在感染当天开始用维生素D3治疗8剂；（第三组）正常对照组；（第四组a）接受维生素D3治疗12剂；（第四组b）接受维生素D3治疗8剂。感染后35天处死小鼠，进行总肌肉幼虫计数，并对肌肉样本进行组织病理学检查，同时对环氧合酶2（COX2）和诱导型一氧化氮合酶（iNOS）进行免疫组化染色。评估肌肉中相对cathelicidin mRNA表达，以及血清中肌肉酶CK和LDH、白细胞介素-4（IL-4）、IL-10、IL-17和干扰素-γ（INF-γ）的水平。补充维生素D3可显著减少肌肉幼虫计数、炎性细胞浸润、COX2和iNOS表达。此外，它增加了cathelicidin基因表达，降低了血清CK和LDH水平，并影响血清细胞因子水平，增加血清IL-4和IL-10水平，同时降低血清INF-γ和IL-17水平。总之，补充维生素D3对旋毛虫病的肌肉期有良好效果，包括抗炎、抗氧化和免疫调节作用。
