Zhao Lili, Dou Qingnan, Chen Shiyue, Wang Yinbin, Yang Qingxiang, Chen Wanrong, Zhang Hao, Du Yirong, Xie Mengfei
College of Life Sciences, Henan Normal University, Xinxiang, 453007, Henan, China; Henan International Joint Laboratory of Agricultural Microbial Ecology and Technology, Henan Normal University, Xinxiang, 453007, China.
College of Life Sciences, Henan Normal University, Xinxiang, 453007, Henan, China.
Chemosphere. 2023 Apr;320:138038. doi: 10.1016/j.chemosphere.2023.138038. Epub 2023 Feb 1.
As a new type of pollutants, nanoplastics (NPs), which are easily ingested by humans from food wraps, salt, drinking water, have been widely detected in various water environments, and are a threat to human health. It is therefore urgent to develop an efficient method to remove NPs from the diet or relief its harm. In the present study, the possibility of a well-known human probiotic, lactic acid bacteria (LAB), was evaluated to remove NPs from food as an absorbent. The results indicated that LAB from infant feces could efficiently absorb three types NPs, i.e. polypropylene (PP), polyethylene (PE), and polyvinyl chloride (PVC) with the adsorption rates of PP > PE > PVC (PP 78.57%, PE 71.59%, PVC 66.57%) and the Nile red-stained NPs being aggregated on the surfaces of Lactobacillus cells. The smaller the particle size, the stronger the ability of NP adsorption on the cell surface. The hydrophobicity of NPs and bacterial cells affected the adsorption process. The measurement of adsorption rates of different cell components indicated that the overall adsorption effect of cell was better than that of individual cell component. The results of molecular dynamics analysis revealed that adsorption was mainly caused by electrostatic interactions, van der Waals forces, and hydrogen bonds. The hydrophobic interaction was also involved in adsorption process. Overall, this research may provide new information for developing new strategies for NPs removal in intestinal environment.
作为一种新型污染物，纳米塑料（NPs）很容易通过食品包装、盐、饮用水等被人类摄入，已在各种水环境中被广泛检测到，对人类健康构成威胁。因此，迫切需要开发一种有效的方法来从饮食中去除纳米塑料或减轻其危害。在本研究中，评估了一种著名的人类益生菌——乳酸菌（LAB）作为吸附剂从食物中去除纳米塑料的可能性。结果表明，来自婴儿粪便的乳酸菌能够有效吸附三种类型的纳米塑料，即聚丙烯（PP）、聚乙烯（PE）和聚氯乙烯（PVC），吸附率为PP > PE > PVC（PP 78.57%，PE 71.59%，PVC 66.57%），尼罗红染色的纳米塑料聚集在乳酸杆菌细胞表面。粒径越小，纳米塑料在细胞表面的吸附能力越强。纳米塑料和细菌细胞的疏水性影响吸附过程。不同细胞成分吸附率的测定表明，细胞的整体吸附效果优于单个细胞成分。分子动力学分析结果表明，吸附主要由静电相互作用、范德华力和氢键引起。疏水相互作用也参与了吸附过程。总体而言，本研究可能为开发肠道环境中纳米塑料去除的新策略提供新信息。
