CgBlimp-1 抑制牡蛎 Crassostrea gigas 免疫反应中的粒细胞增殖和白细胞介素产生。
CgBlimp-1 inhibits granulocytes proliferation and interleukin production in the immune response of oyster Crassostrea gigas.
机构信息
Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, 116023, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China.
Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, 116023, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China.
出版信息
Dev Comp Immunol. 2023 May;142:104652. doi: 10.1016/j.dci.2023.104652. Epub 2023 Feb 1.
B lymphocyte-inducible maturation protein 1 (Blimp-1) is a SET domain and zinc fingers containing transcriptional repressor, which is necessary for regulating the development of many immune cell lineages and keeping immune homeostasis. In the present study, a Blimp-1 homologue (designated as CgBlimp-1) was identified from oyster Crassostrea gigas, which contained a conserved SET domain and five ZnF_C2H2 domains and shared high homology with Blimp-1 from other species. The mRNA transcripts of CgBlimp-1 were highly expressed in gill and hepatopancreas. CgBlimp-1 protein was detected to be specifically expressed in granulocytes. After V. splendidus stimulation, the mRNA expression level of CgBlimp-1 in haemocytes up-regulated significantly at 24, 48, and 96 h, which was 4.39-fold (p < 0.05), 7.68-fold (p < 0.01) and 2.65-fold (p < 0.05) of that in control group, respectively. When the expression of CgBlimp-1 was knocked-down in vivo by RNAi, the mRNA expressions of downstream transcription factor CgMyc-A (1.63-fold of that in control group, p < 0.05) and cell cycle related gene CgCDK2 (1.70-fold, p < 0.05) increased significantly at 24 h after V. splendidus stimulation. Concomitantly, the ratio of EdU haemocytes increased notably (p < 0.01) while the proportion of haemocytes in G0/G1 phase decreased dramatically (p < 0.001), compared to that in control group. More specifically, the proportion of granulocytes in total haemocytes increased apparently (p < 0.05) in CgBlimp-1-RNAi oysters, together with up-regulation (p < 0.05) of the ratio of EdU granulocytes and down-regulation (p < 0.001) of the proportion of granulocytes in G0/G1 phase. Furthermore, the mRNA expression levels of CgIL17-1, CgIL17-2 and CgIL17-4 in haemocytes increased significantly in CgBlimp-1-RNAi oysters, which was 1.71-fold (p < 0.05), 144.70-fold (p < 0.01) and 1.93-fold (p < 0.05) of that in control group, respectively. Aforementioned results suggested that CgBlimp-1 could reduce the proliferation of granulocytes by arresting cell cycle in G1/G0 phase and avoid over-expression of interleukin to maintain homeostasis in the immune response of oyster.
B 淋巴细胞诱导成熟蛋白 1（Blimp-1）是一种 SET 结构域和锌指结构域含有转录抑制因子，它对于调节许多免疫细胞谱系的发育和维持免疫稳态是必要的。本研究从牡蛎（Crassostrea gigas）中鉴定出一种 Blimp-1 同源物（命名为 CgBlimp-1），它含有一个保守的 SET 结构域和五个 ZnF_C2H2 结构域，与其他物种的 Blimp-1 具有高度同源性。CgBlimp-1 的 mRNA 转录本在鳃和肝胰腺中高度表达。CgBlimp-1 蛋白在粒细胞中特异性表达。在灿烂弧菌刺激后，血细胞中 CgBlimp-1 的 mRNA 表达水平在 24、48 和 96 h 时显著上调，分别为对照组的 4.39 倍（p<0.05）、7.68 倍（p<0.01）和 2.65 倍（p<0.05）。体内通过 RNAi 敲低 CgBlimp-1 的表达后，下游转录因子 CgMyc-A（对照组的 1.63 倍，p<0.05）和细胞周期相关基因 CgCDK2（1.70 倍，p<0.05）的 mRNA 表达在灿烂弧菌刺激后 24 h 时显著增加。同时，与对照组相比，EdU 血细胞的比例显著增加（p<0.01），而 G0/G1 期血细胞的比例显著降低（p<0.001）。更具体地说，在 CgBlimp-1-RNAi 牡蛎中，总血细胞中粒细胞的比例明显增加（p<0.05），同时 EdU 粒细胞的比例增加（p<0.05），G0/G1 期粒细胞的比例降低（p<0.001）。此外，CgBlimp-1-RNAi 牡蛎中血细胞中 CgIL17-1、CgIL17-2 和 CgIL17-4 的 mRNA 表达水平分别显著上调，分别为对照组的 1.71 倍（p<0.05）、144.70 倍（p<0.01）和 1.93 倍（p<0.05）。上述结果表明，CgBlimp-1 可以通过将细胞周期阻滞在 G1/G0 期来减少粒细胞的增殖，并避免白细胞介素的过度表达，从而维持牡蛎免疫反应的稳态。
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