Department of Comparative Biochemistry and Bioanalytics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
Yeast. 2023 Aug;40(8):349-359. doi: 10.1002/yea.3842. Epub 2023 Feb 13.
Candida albicans is one of the main pathogens responsible for the development of difficult-to-fight fungal infections called candidiasis. Neutrophils are the major effector cells involved in the eradication of fungal pathogens. This group of immune cells uses several mechanisms that enable the rapid neutralization of pathogens. The most frequently identified mechanisms are phagocytosis and the release of neutrophil extracellular traps (NETs). The mechanism for selecting the type of neutrophil immune response is still unknown. In our study, we analyzed the relationship between the activation of phagocytosis and netosis. We detected the presence of two neutrophil populations characterized by different response patterns to contact with C. albicans blastospores. The first neutrophil population showed an increased ability to rapidly release NETs without prior internalization of the pathogen. In the second population, the netosis process was inherently associated with phagocytosis. Differences between populations also referred to the production of reactive oxygen species. Our results suggest that neutrophils use different strategies to fight C. albicans and, contrary to previous reports, these mechanisms are not mutually exclusive.
白色念珠菌是导致真菌感染（又称念珠菌病）的主要病原体之一。中性粒细胞是参与消除真菌病原体的主要效应细胞。这群免疫细胞利用几种机制实现对病原体的快速中和。最常被识别的机制是吞噬作用和中性粒细胞胞外陷阱（NETs）的释放。选择中性粒细胞免疫反应类型的机制尚不清楚。在我们的研究中，我们分析了吞噬作用和网脱作用激活之间的关系。我们检测到两种具有不同反应模式的中性粒细胞群体的存在，它们与与白念珠菌芽生孢子接触有关。第一群中性粒细胞表现出快速释放 NETs 的能力增强，而无需先前内化病原体。在第二群体中，网脱过程本质上与吞噬作用有关。群体之间的差异还涉及活性氧物质的产生。我们的结果表明，中性粒细胞使用不同的策略来对抗白色念珠菌，与之前的报道相反，这些机制并非相互排斥。
