Infectious Disease Program, Lovelace Biomedical Research Institute, Albuquerque, NM, USA.
Infectious Disease Program, Lovelace Biomedical Research Institute, Albuquerque, NM, USA.
Semin Cell Dev Biol. 2023 Sep 15;146:40-56. doi: 10.1016/j.semcdb.2023.01.013. Epub 2023 Feb 2.
Alternative RNA splicing is a co-transcriptional process that richly increases proteome diversity, and is dynamically regulated based on cell species, lineage, and activation state. Virus infection in vertebrate hosts results in rapid host transcriptome-wide changes, and regulation of alternative splicing can direct a combinatorial effect on the host transcriptome. There has been a recent increase in genome-wide studies evaluating host alternative splicing during viral infection, which integrates well with prior knowledge on viral interactions with host splicing proteins. A critical challenge remains in linking how these individual events direct global changes, and whether alternative splicing is an overall favorable pathway for fending off or supporting viral infection. Here, we introduce the process of alternative splicing, discuss how to analyze splice regulation, and detail studies on genome-wide and splice factor changes during viral infection. We seek to highlight where the field can focus on moving forward, and how incorporation of a virus-host co-evolutionary perspective can benefit this burgeoning subject.
可变剪接是一种共转录过程，可极大地增加蛋白质组的多样性，并根据细胞种类、谱系和激活状态进行动态调节。脊椎动物宿主中的病毒感染会导致宿主转录组的广泛快速变化，而可变剪接的调节可以直接对宿主转录组产生组合效应。最近，越来越多的全基因组研究评估了病毒感染过程中的宿主可变剪接，这与先前关于病毒与宿主剪接蛋白相互作用的知识很好地结合在一起。一个关键的挑战仍然是如何将这些单个事件联系起来，以及可变剪接是否是抵御或支持病毒感染的整体有利途径。在这里，我们介绍了可变剪接的过程，讨论了如何分析剪接调控，并详细介绍了病毒感染过程中全基因组和剪接因子变化的研究。我们试图强调该领域可以关注的重点，以及纳入病毒-宿主共同进化的观点如何使这个新兴课题受益。
