Yu Songfeng, Wang Wenjun, Bu Tingting, Zhao Runan, Niu Ruihao, Liu Ling, Li Jiaheng, Wu Jianping, Liu Donghong
College of Biosystems Engineering and Food Science, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang Engineering Laboratory of Food Technology and Equipment, Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China.
College of Biosystems Engineering and Food Science, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang Engineering Laboratory of Food Technology and Equipment, Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China; Innovation Center of Yangtze River Delta, Zhejiang University, Jiashan 314100, China.
Food Res Int. 2023 Feb;164:112340. doi: 10.1016/j.foodres.2022.112340. Epub 2022 Dec 20.
Two novel hypoglycemic peptides VY and SFLLR were identified from douchi as the major peptides responsible for the glucose uptake activity. The present work aimed to elucidate their digestion, absorption and transport properties using simulated digestion and Caco-2 cell monolayers transport models. Besides, the effects of digestion and absorption on the structure and activity were also studied. The results showed that VY was resistant to gastrointestinal tract digestion and could cross Caco-2 cell monolayers intactly via both TJs-mediated passive paracellular pathway and PepT1-mediated active route. In comparison, SFLLR was partially degraded into small fragments of SFLL, SFL, and SF by the digestive system, leading to increased glucose uptake activity. Notably, SFLLR, SFLL, and SFL were partly hydrolyzed by aminopeptidase N or dipeptidyl peptidase IV during transport, but they were transported intact. SFL was transported via both paracellular diffusion and PepT1-mediated routes, while SFLLR and SFLL were via paracellular route only.
从豆豉中鉴定出两种新型降血糖肽VY和SFLLR，它们是负责葡萄糖摄取活性的主要肽。本研究旨在利用模拟消化和Caco-2细胞单层转运模型阐明它们的消化、吸收和转运特性。此外，还研究了消化和吸收对其结构和活性的影响。结果表明，VY对胃肠道消化具有抗性，并且可以通过紧密连接（TJs）介导的被动细胞旁途径和肽转运体1（PepT1）介导的主动途径完整地穿过Caco-2细胞单层。相比之下，SFLLR被消化系统部分降解为SFLL、SFL和SF的小片段，导致葡萄糖摄取活性增加。值得注意的是，SFLLR、SFLL和SFL在转运过程中被氨肽酶N或二肽基肽酶IV部分水解，但它们仍能完整转运。SFL通过细胞旁扩散和PepT1介导的途径进行转运，而SFLLR和SFLL仅通过细胞旁途径进行转运。
