Jiangsu Province Engineering Research Center of Food Synthetic Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; Science Center for Future Foods, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
Jiangsu Province Engineering Research Center of Food Synthetic Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; Science Center for Future Foods, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
Food Res Int. 2023 Feb;164:112392. doi: 10.1016/j.foodres.2022.112392. Epub 2022 Dec 27.
As a novel animal meat alternative, plant-based meat (PBM) frequently suffers from quality problems as a result of freeze-thaw cycles in commercial transportation and household storage. There is a need to reduce the deterioration of PBM attributes, such as water holding capacity, as a result of these freeze-thaw cycles. In this study, Daucus carota antifreeze protein (DcAFP) and its deglycosylated mutant DcAFP-N294G were heterologously expressed in Komagataella phaffii X33. The effects of pretreatment with recombinant AFPs (rAFPs) on the microstructure, rheological properties, water mobility, and water distribution of PBM were assessed. The rDcAFP-N294G-treated PBM samples had superior viscoelasticity and water distribution features compared to the rDcAFP-treated group because the complex N-linked oligosaccharides did not interfere with the binding of rAFPs to ice molecules. In addition, rAFP pretreatment resulted in a smoother and flatter surface of the high-moisture protein extrudate matrix compared to the commercial cryoprotectant trehalose. Deglycosylated DcAFP has potential applications as a new effective cryoprotectant in meat alternatives.
作为一种新型的动物肉类替代品，植物基肉（PBM）在商业运输和家庭储存过程中经常因冻融循环而出现质量问题。需要减少 PBM 属性的恶化，例如水保持能力，由于这些冻融循环。在这项研究中，胡萝卜抗冻蛋白（DcAFP）及其去糖基化突变体 DcAFP-N294G 在毕赤酵母 X33 中异源表达。评估了重组 AFP（rAFP）预处理对 PBM 微观结构、流变特性、水分迁移率和水分分布的影响。与 rDcAFP 处理组相比，rDcAFP-N294G 处理的 PBM 样品具有更好的粘弹性和水分分布特征，因为复杂的 N 连接寡糖不会干扰 rAFP 与冰分子的结合。此外，与商业冷冻保护剂海藻糖相比，rAFP 预处理导致高水分蛋白挤出基质的表面更光滑、更平坦。去糖基化的 DcAFP 具有作为肉类替代品的新型有效冷冻保护剂的潜力。
