硫酸鱼精蛋白稳定的硒纳米粒子的合成、表征及抗癌活性。
Synthesis, characterization, and anticancer activity of protamine sulfate stabilized selenium nanoparticles.
机构信息
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu, China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu, China.
出版信息
Food Res Int. 2023 Feb;164:112435. doi: 10.1016/j.foodres.2022.112435. Epub 2022 Dec 31.
Selenium nanoparticles (SeNPs) have attracted much recent interest as nutraceuticals, while they face great challenges, such as poor stability and low cellular uptake efficiency. This study introduced a facile approach to synthesizing protamine sulfate (PS) functionalized selenium nanoparticles (PS-SeNPs) by using PS as a surface decorator. The monodisperse spherical PS-SeNPs with a particle size of 130 nm and a ζ-potential of +31 mV were ligated with PS through Se-N, Se-O bonds, and physical adsorption, which exhibits excellent physical stability against pH, temperature, and storage time. The positive surface charge of PS-SeNPs contributed to the enhancement of cellular uptake efficiency by endocytosis, which was 3-times higher than bare SeNPs. Compared to SeNPs (IC = 17.675 μg/mL), PS-SeNPs could dramatically inhibit the proliferation of HepG2 cells with an IC value of 5.507 μg/mL, as reflected by the induction of apoptosis, S phase arresting, overproduction of intracellular ROS, and depolarization of mitochondria membrane. Overall, these results demonstrated the great potential of PS-SeNPs that can be applied as a functional ingredient in foods and nutraceuticals.
硒纳米颗粒（SeNPs）作为营养保健品受到了广泛关注，但它们面临着稳定性差、细胞摄取效率低等挑战。本研究采用一种简便的方法，通过使用鱼精蛋白（PS）作为表面修饰剂来合成硫酸鱼精蛋白（PS）功能化的硒纳米颗粒（PS-SeNPs）。PS-SeNPs 呈单分散球形，粒径为 130nm，ζ-电位为+31mV，通过 Se-N、Se-O 键和物理吸附与 PS 连接，表现出对 pH、温度和储存时间的优异物理稳定性。PS-SeNPs 的正表面电荷有助于通过内吞作用提高细胞摄取效率，是 bare SeNPs 的 3 倍。与 SeNPs（IC=17.675μg/mL）相比，PS-SeNPs 可显著抑制 HepG2 细胞的增殖，IC 值为 5.507μg/mL，这反映在细胞凋亡、S 期阻滞、细胞内 ROS 过度产生和线粒体膜去极化的诱导上。总的来说，这些结果表明 PS-SeNPs 具有很大的应用潜力，可作为食品和营养保健品中的功能性成分。
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